Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of COLO 320 using 16818-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 16818-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16818-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 16818-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16818-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 16818-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16818-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of PC-3 using 16818-1-AP
IP result of anti-EED (IP:16818-1-AP, 4ug; Detection:16818-1-AP 1:500) with PC-3 cells lysate 3200ug.
IHC staining of human tonsillitis using 16818-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 16818-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16818-1-AP
Immunofluorescent analysis of (10% Formalin) fixed HepG2 cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 16818-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 16818-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EED (16818-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

EED Polyclonal antibody

EED Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16818-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

WAIT-1, WAIT1, WAIT 1, Polycomb protein EED, Embryonic ectoderm development protein

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, A431 cells, HeLa cells, HepG2 cells, K-562 cells, PC-3 cells
Positive IP detected in	PC-3 cells
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 19 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

16818-1-AP targets EED in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EED fusion protein Ag10358 相同性解析による交差性が予測される生物種
Full Name	embryonic ectoderm development
Calculated molecular weight	466 aa, 53 kDa
Observed molecular weight	53 kDa
GenBank accession number	BC068995
Gene Symbol	EED
Gene ID (NCBI)	8726
RRID	AB_2262065
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75530
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

Protocols

Product Specific Protocols
WB protocol for EED antibody 16818-1-AP	Download protocol
IHC protocol for EED antibody 16818-1-AP	Download protocol
IF protocol for EED antibody 16818-1-AP	Download protocol
IP protocol for EED antibody 16818-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer Authors - Rashmi Bharti View Article
human	WB	Blood Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia Authors - Xinyue Zhou View Article
human	WB	EMBO J A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination. Authors - Xu Wang View Article
human	WB	Eur J Med Chem Discovery of precision targeting EZH2 degraders for triple-negative breast cancer. Authors - Cheng Wang View Article
		J Cell Sci EZH1/SUZ12 complex positively regulates the transcription of NF-κB target genes via interaction with UXT. Authors - Shuai-Kun Su View Article
human	WB	Sci Rep Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1. Authors - Masamitsu N Asaka View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of COLO 320 using 16818-1-AP

WB analysis of PC-3 using 16818-1-AP

WB analysis of K-562 using 16818-1-AP

WB analysis of A431 using 16818-1-AP

WB analysis of HepG2 using 16818-1-AP

WB analysis of COLO 320 using 16818-1-AP

WB analysis of HeLa using 16818-1-AP

IP experiment of PC-3 using 16818-1-AP

IHC staining of human tonsillitis using 16818-1-AP

IHC staining of human tonsillitis using 16818-1-AP

IF Staining of HepG2 using 16818-1-AP

IF Staining of HeLa using 16818-1-AP

FC experiment of HepG2 using 16818-1-AP

EED Polyclonal antibody

EED Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer

Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.

EZH1/SUZ12 complex positively regulates the transcription of NF-κB target genes via interaction with UXT.

Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.