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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EEF1A1 Monoclonal antibody, PBS Only

EEF1A1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A9F5

Cat no : 67495-1-PBS

Synonyms

EEF1A, EEF 1, EC:3.6.5.-, CCS3, CCS 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EEF1A1 Monoclonal antibody (67495-1-Ig)

    WB analysis using 67495-1-Ig (same clone as 67495-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67495-1-Ig (EEF1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using EEF1A1 Monoclonal antibody (67495-1-Ig)

    WB analysis of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

    WB result of EEF1A1 antibody (67495-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EEF1A1 Monoclonal antibody (67495-1-Ig)

    IHC staining of human pancreas cancer using 67495-1-Ig (same clone as 67495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EEF1A1 Monoclonal antibody (67495-1-Ig)

    IHC staining of mouse brain using 67495-1-Ig (same clone as 67495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EEF1A1 Monoclonal antibody (67495-1-Ig)

    IHC staining of mouse brain using 67495-1-Ig (same clone as 67495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EEF1A1 Monoclonal antibody (67495-1-Ig)

    IHC staining of human pancreas cancer using 67495-1-Ig (same clone as 67495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EEF1A1 Monoclonal antibody (67495-1-Ig)

    IF Staining of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67495-1-Ig (EEF1A1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using EEF1A1 Monoclonal antibody (67495-1-Ig)

    FC experiment of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EEF1A1 (67495-1-Ig, Clone:1A9F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67495-1-PBS targets EEF1A1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF1A1 fusion protein Ag1938 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 alpha 1
Calculated molecular weight 462 aa, 50 kDa
Observed molecular weight49 kDa
GenBank accession numberBC014224
Gene symbol EEF1A1
Gene ID (NCBI) 1915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.