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  • Featured Product
  • KD/KO Validated

EEF1A1 Recombinant antibody

EEF1A1 Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2B16

Cat no : 81377-1-RR

Synonyms

EEF1A, EEF 1, EC:3.6.5.-, CCS3, CCS 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using EEF1A1 Recombinant antibody (81377-1-RR)

    WB analysis of HepG2 using 81377-1-RR

    WB result of EEF1A1 antibody (81377-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1A1 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using EEF1A1 Recombinant antibody (81377-1-RR)

    WB analysis using 81377-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81377-1-RR (EEF1A1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using EEF1A1 Recombinant antibody (81377-1-RR)

    IP experiment of HeLa using 81377-1-RR

    IP result of anti-EEF1A1 (IP:81377-1-RR, 4ug; Detection:81377-1-RR 1:8000) with HeLa cells lysate 1720 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EEF1A1 Recombinant antibody (81377-1-RR)

    IHC staining of human pancreas cancer using 81377-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using EEF1A1 Recombinant antibody (81377-1-RR)

    IHC staining of human colon using 81377-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EEF1A1 Recombinant antibody (81377-1-RR)

    IHC staining of human pancreas cancer using 81377-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EEF1A1 Recombinant antibody (81377-1-RR)

    IHC staining of mouse brain using 81377-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EEF1A1 Recombinant antibody (81377-1-RR)

    IHC staining of mouse brain using 81377-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EEF1A1 Recombinant antibody (81377-1-RR)

    IF Staining of HepG2 using 81377-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EEF1A1 antibody (81377-1-RR, Clone: 2B16 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EEF1A1 Recombinant antibody (81377-1-RR)

    IF Staining of HepG2 using 81377-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EEF1A1 antibody (81377-1-RR, Clone: 2B16 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81377-1-RR

    Affinity and Kinetic Characterization of 81377-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81377-1-RR against Human EEF1A1 were performed. The affinity constant is 25.3 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human pancreas cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81377-1-RR targets EEF1A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EEF1A1 fusion protein Ag1938 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 alpha 1
Calculated molecular weight 462 aa, 50 kDa
Observed molecular weight50 kDa
GenBank accession numberBC014224
Gene symbol EEF1A1
Gene ID (NCBI) 1915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.1 It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.

Protocols

Product Specific Protocols
WB protocol for EEF1A1 antibody 81377-1-RRDownload protocol
IHC protocol for EEF1A1 antibody 81377-1-RRDownload protocol
IF protocol for EEF1A1 antibody 81377-1-RRDownload protocol
IP protocol for EEF1A1 antibody 81377-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols