EEF1A2 Monoclonal antibody, PBS Only
EEF1A2 Monoclonal Antibody for WB, Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig
Applications
WB, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
4F3F4
Cat no : 66806-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
66806-1-PBS targets EEF1A2 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EEF1A2 fusion protein Ag6875 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation elongation factor 1 alpha 2 |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC000432 |
| Gene symbol | EEF1A2 |
| Gene ID (NCBI) | 1917 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
eEF1A2 is one of two isoforms (eEF1A1 and eEF1A2) of eukaryotic elongation factor 1 alpha (eEF1A or eEF1α). The two isoforms share more than 90% sequence identity and have the similar function that being a protein translation factor involved in protein synthesis. Addition, eEF1A2 plays an important role in cell cycle regulation, heat-shock response, aging, posttranslational modifications, and signal transduction (PMID: 25905039). eEF1A2 is expressed in heart, brain tissue, diaphragm and skeletal muscle while eEF1A1 is expressed ubiquitously (PMID:14588074). And the eEF1A2 protein stimulates the phospholipid signaling and activates the Akt-dependent cell migration and actin remodeling that ultimately favors tumorigenesis. It is reported that eEF1A2 was related with kinds of cancers such as ovarian cancer, prostate cancer, pancreatic cancer, breast cancer and lung cancer (PMID:14588074; 24853801; 25744894; 25905039). An 48 kDa band has also been reported(PMID: 24285179).
