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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EEF1A2 Monoclonal antibody (66806-1-Ig)

    WB analysis using 66806-1-Ig (same clone as 66806-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using EEF1A2 Monoclonal antibody (66806-1-Ig)

    WB analysis of mouse brain using 66806-1-Ig (same clone as 66806-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using EEF1A2 Monoclonal antibody (66806-1-Ig)

    WB analysis of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using EEF1A2 Monoclonal antibody (66806-1-Ig)

    WB analysis of pig brain using 66806-1-Ig (same clone as 66806-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using EEF1A2 Monoclonal antibody (66806-1-Ig)

    IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66806-1-Ig (EEF1A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using EEF1A2 Monoclonal antibody (66806-1-Ig)

    IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66806-1-Ig (EEF1A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

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EEF1A2 Monoclonal antibody, PBS Only

EEF1A2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66806-1-PBS
Clone No.4F3F4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

Eukaryotic elongation factor 1 A-2, Elongation factor 1-alpha 2, EF-1-alpha-2, EEF1AL, eEF1A-2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66806-1-PBS targets EEF1A2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF1A2 fusion protein Ag6875 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 alpha 2
Calculated molecular weight 50 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC000432
Gene Symbol EEF1A2
Gene ID (NCBI) 1917
RRIDAB_2882149
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ05639
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

eEF1A2 is one of two isoforms (eEF1A1 and eEF1A2) of eukaryotic elongation factor 1 alpha (eEF1A or eEF1α). The two isoforms share more than 90% sequence identity and have the similar function that being a protein translation factor involved in protein synthesis. Addition, eEF1A2 plays an important role in cell cycle regulation, heat-shock response, aging, posttranslational modifications, and signal transduction (PMID: 25905039). eEF1A2 is expressed in heart, brain tissue, diaphragm and skeletal muscle while eEF1A1 is expressed ubiquitously (PMID:14588074). And the eEF1A2 protein stimulates the phospholipid signaling and activates the Akt-dependent cell migration and actin remodeling that ultimately favors tumorigenesis. It is reported that eEF1A2 was related with kinds of cancers such as ovarian cancer, prostate cancer, pancreatic cancer, breast cancer and lung cancer (PMID:14588074; 24853801; 25744894; 25905039). An 48 kDa band has also been reported(PMID: 24285179).