Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 10483-1-AP
WB result of EEF1B2 antibody (10483-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells.
WB analysis of PC-3 using 10483-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10483-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10483-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10483-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10483-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10483-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10483-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10483-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10483-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 10483-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10483-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 10483-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10483-1-AP (EEF1B2 antibody) at dilution of 1:100 (under 25x lens).

Featured Product
KD/KO Validated

EEF1B2 Polyclonal antibody

EEF1B2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10483-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	PC-3 cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, SKOV-3 cells, mouse kidney tissue
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

10483-1-AP targets EEF1B2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EEF1B2 fusion protein Ag0742 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation elongation factor 1 beta 2
Calculated molecular weight	29 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC004931
Gene Symbol	EEF1B2
Gene ID (NCBI)	1933
RRID	AB_2096985
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P24534
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against full length EEF1B2 of human origin.

Protocols

Product Specific Protocols
WB protocol for EEF1B2 antibody 10483-1-AP	Download protocol
IHC protocol for EEF1B2 antibody 10483-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	PLoS Genet Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors. Authors - Emily M Schleicher KD Validated View Article
human	WB,IF,IP	Mol Cell Biol Translational repression by RNA-binding protein TIAR. Authors - Mazan-Mamczarz Krystyna K View Article
mouse	WB	Front Mol Biosci Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1. Authors - Taku Kaitsuka View Article
rat	WB	Proteomics Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver. Authors - Fofana Bourlaye B View Article
human	WB	Amino Acids Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA. Authors - Yusuke Terui View Article
human,mouse	WB	PLoS One Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery. Authors - Pitteri Sharon J SJ View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10483-1-AP

WB analysis of PC-3 using 10483-1-AP

WB analysis of HepG2 using 10483-1-AP

WB analysis of mouse kidney using 10483-1-AP

WB analysis of HEK-293 using 10483-1-AP

WB analysis of HEK-293 using 10483-1-AP

WB analysis of PC-3 using 10483-1-AP

WB analysis of HeLa using 10483-1-AP

WB analysis of Jurkat using 10483-1-AP

WB analysis of HepG2 using 10483-1-AP

WB analysis of SKOV-3 using 10483-1-AP

WB analysis of HepG2 using 10483-1-AP

IHC staining of human pancreas cancer using 10483-1-AP

EEF1B2 Polyclonal antibody

EEF1B2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

Translational repression by RNA-binding protein TIAR.

Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.

Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver.

Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA.

Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.