Various lysates were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

WB result of EEF1B2 antibody (60329-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

HeLa cells were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60329-1-Ig (EEF1B2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60329-1-Ig(EEF1B2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.