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  • Featured Product
  • KD/KO Validated

EEF1D Monoclonal antibody

EEF1D Monoclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G4C4

Cat no : 60085-2-Ig

Synonyms

Antigen NY CO 4, EEF1D, EF 1 delta, EF 1D, EF1D, Elongation factor 1 delta, FP1047

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of MCF-7 using 60085-2-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of Jurkat using 60085-2-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of NIH/3T3 using 60085-2-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of HeLa using 60085-2-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of RAW 264.7 using 60085-2-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    IP experiment of MCF-7 using 60085-2-Ig

    IP result of anti-EEF1D (IP:60085-2-Ig, 5ug; Detection:60085-2-Ig 1:1000) with MCF-7 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EEF1D Monoclonal antibody (60085-2-Ig)

    IHC staining of human pancreas cancer using 60085-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EEF1D Monoclonal antibody (60085-2-Ig)

    IHC staining of human pancreas cancer using 60085-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50.

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Tested Applications

Positive WB detected intransfection MCF-7 cells, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60085-2-Ig targets EEF1D in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF1D fusion protein Ag0983 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Calculated molecular weight 31 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC007847
Gene symbol EEF1D
Gene ID (NCBI) 1936
RRIDAB_2096994
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Protocols

Product Specific Protocols
WB protocol for EEF1D antibody 60085-2-IgDownload protocol
IHC protocol for EEF1D antibody 60085-2-IgDownload protocol
IP protocol for EEF1D antibody 60085-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Virol

Eukaryotic translation elongation factor 1 delta inhibits the nuclear import of nucleoprotein and PA-PB1 heterodimer of influenza A virus.

Authors - Qingxia Gao
  • KO Validated
View Article