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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EEF1G Monoclonal antibody

EEF1G Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G7C6

Cat no : 68148-1-Ig

Synonyms

eEF 1B gamma, EEF1G, EF 1 gamma, EF1G, Elongation factor 1 gamma, GIG35

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EEF1G Monoclonal antibody (68148-1-Ig)

    WB analysis using 68148-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EEF1G Monoclonal antibody (68148-1-Ig)

    WB analysis using 68148-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EEF1G Monoclonal antibody (68148-1-Ig)

    WB analysis using 68148-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EEF1G Monoclonal antibody (68148-1-Ig)

    WB analysis using 68148-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EEF1G Monoclonal antibody (68148-1-Ig)

    IF Staining of HeLa using 68148-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EEF1G Monoclonal antibody (68148-1-Ig)

    IF Staining of HeLa using 68148-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inU2OS cells, HeLa cells, MCF-7 cells, pig heart tissue, A549 cells, HEK-293 cells, rabbit heart tissue, pig brain tissue, rabbit brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68148-1-Ig targets EEF1G in WB, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rabbit samples.

Tested Reactivity Human, Pig, Rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EEF1G fusion protein Ag0218 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 gamma
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC006520
Gene symbol EEF1G
Gene ID (NCBI) 1937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

Protocols

Product Specific Protocols
WB protocol for EEF1G antibody 68148-1-IgDownload protocol
IF protocol for EEF1G antibody 68148-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols