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EEF2 Polyclonal antibody

Name: EEF2 Polyclonal antibody
Brand: Proteintech
SKU: 20107-1-AP

EEF2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20107-1-AP

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 20107-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20107-1-AP
WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HEK-293 cells.
WB analysis of HeLa using 20107-1-AP
WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells.
WB analysis of NIH/3T3 using 20107-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 20107-1-AP
IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug.
IHC staining of human stomach cancer using 20107-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human stomach cancer using 20107-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human normal colon using 20107-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 20107-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 20107-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 20107-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
FC experiment of HeLa using 20107-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 20107-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	C6 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IP	See 1 publications below
CoIP	See 1 publications below
RIP	See 2 publications below

Product Information

20107-1-AP targets EEF2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EEF2 fusion protein Ag13826 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation elongation factor 2
Calculated molecular weight	858 aa, 95 kDa
Observed molecular weight	95-100 kDa
GenBank accession number	BC126259
Gene symbol	EEF2
Gene ID (NCBI)	1938
RRID	AB_10950401
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for EEF2 antibody 20107-1-AP	Download protocol
IHC protocol for EEF2 antibody 20107-1-AP	Download protocol
IF protocol for EEF2 antibody 20107-1-AP	Download protocol
IP protocol for EEF2 antibody 20107-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
zebrafish	WB	Nature A molecular network of conserved factors keeps ribosomes dormant in the egg Authors - Friederike Leesch View Article
human	WB	Cancer Lett eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells. Authors - Cheng Ji View Article
human	WB,CoIP,RIP	Carcinogenesis PLAGL2 promotes Snail expression and gastric cancer progression via UCA1/miR-145-5p/YTHDF1 axis Authors - Wen Chen View Article
human	WB	Biomed Microdevices Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria. Authors - Hao-Peng Lin View Article
human	WB	Pathol Res Pract miR-183-5p acts as a potential prognostic biomarker in gastric cancer and regulates cell functions by modulating EEF2. Authors - Wang Li View Article
human	WB	BMC Nephrol Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells. Authors - Yang Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EEF2 Polyclonal antibody

EEF2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 20107-1-AP

WB analysis of HEK-293 using 20107-1-AP

WB analysis of HeLa using 20107-1-AP

WB analysis of NIH/3T3 using 20107-1-AP

IP experiment of HeLa using 20107-1-AP

IHC staining of human stomach cancer using 20107-1-AP

IHC staining of human stomach cancer using 20107-1-AP

IHC staining of human normal colon using 20107-1-AP

IHC staining of human breast cancer using 20107-1-AP

IHC staining of human breast cancer using 20107-1-AP

IF Staining of HepG2 using 20107-1-AP

FC experiment of HeLa using 20107-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A molecular network of conserved factors keeps ribosomes dormant in the egg

eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.

PLAGL2 promotes Snail expression and gastric cancer progression via UCA1/miR-145-5p/YTHDF1 axis

Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.

miR-183-5p acts as a potential prognostic biomarker in gastric cancer and regulates cell functions by modulating EEF2.

Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.

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