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KD/KO Validated

eEF2K Polyclonal antibody

Name: eEF2K Polyclonal antibody
Brand: Proteintech
SKU: 13510-1-AP

eEF2K Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13510-1-AP

Synonyms

eEF-2K, eEF-2 kinase, eEF 2K, eEF 2 kinase, EC:2.7.11.20

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of Jurkat using 13510-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13510-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 13510-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of PC-3 using 13510-1-AP
IP result of anti-eEF2K (IP:13510-1-AP, 4ug; Detection:13510-1-AP 1:500) with PC-3 cells lysate 3200ug.
IF Staining of PC-3 using 13510-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13510-1-AP (EEF2K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13510-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	PC-3 cells, Jurkat cells, HEK-293 cells
Positive IP detected in	PC-3 cells
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

13510-1-AP targets eEF2K in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	eEF2K fusion protein Ag4432 相同性解析による交差性が予測される生物種
Full Name	eukaryotic elongation factor-2 kinase
Calculated molecular weight	725 aa, 100 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC032665
Gene symbol	EEF2K
Gene ID (NCBI)	29904
RRID	AB_2277761
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for eEF2K antibody 13510-1-AP	Download protocol
IF protocol for eEF2K antibody 13510-1-AP	Download protocol
IP protocol for eEF2K antibody 13510-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Lett eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells. Authors - Cheng Ji View Article
human	WB	Oncol Lett AMPK decreases ERK1/2 activity and cancer cell sensitivity to nutrition deprivation by mediating a positive feedback loop involving eEF2K. Authors - Shujuan Tong View Article
rat	WB,IP,CoIP	Cells SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy. Authors - Julie Pires Da Silva KD Validated View Article
human	WB	Med Oncol A drug repurposing study identifies novel FOXM1 inhibitors with in vitro activity against breast cancer cells Authors - Khaled A N Abusharkh View Article
		J Mol Med (Berl) FOXM1 plays a role in autophagy by transcriptionally regulating Beclin-1 and LC3 genes in human triple-negative breast cancer cells. Authors - Zuhal Hamurcu View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

eEF2K Polyclonal antibody

eEF2K Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 13510-1-AP

WB analysis of HEK-293 using 13510-1-AP

WB analysis of PC-3 using 13510-1-AP

IP experiment of PC-3 using 13510-1-AP

IF Staining of PC-3 using 13510-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.

AMPK decreases ERK1/2 activity and cancer cell sensitivity to nutrition deprivation by mediating a positive feedback loop involving eEF2K.

SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy.

A drug repurposing study identifies novel FOXM1 inhibitors with in vitro activity against breast cancer cells

FOXM1 plays a role in autophagy by transcriptionally regulating Beclin-1 and LC3 genes in human triple-negative breast cancer cells.