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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EEFSEC Polyclonal antibody

EEFSEC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10628-1-AP

Synonyms

EEFSEC, EFSEC, Elongation factor sec, SELB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EEFSEC Polyclonal antibody (10628-1-AP)

    WB analysis using 10628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using EEFSEC Polyclonal antibody (10628-1-AP)

    WB analysis of A431 using 10628-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EEFSEC Polyclonal antibody (10628-1-AP)

    WB analysis of HEK-293 using 10628-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EEFSEC Polyclonal antibody (10628-1-AP)

    IHC staining of human kidney using 10628-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using EEFSEC Polyclonal antibody (10628-1-AP)

    IHC staining of human stomach using 10628-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using EEFSEC Polyclonal antibody (10628-1-AP)

    IHC staining of human stomach using 10628-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EEFSEC Polyclonal antibody (10628-1-AP)

    IHC staining of human kidney using 10628-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, A431 cells, HEK-293 cells, Jurkat cells, HepG2 cells
Positive IHC detected inhuman kidney tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10628-1-AP targets EEFSEC in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEFSEC fusion protein Ag0980 相同性解析による交差性が予測される生物種
Full Name eukaryotic elongation factor, selenocysteine-tRNA-specific
Calculated molecular weight 63 kDa
Observed molecular weight65 kDa
GenBank accession numberBC007933
Gene symbol EEFSEC
Gene ID (NCBI) 60678
RRIDAB_2293378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The amino acid selenocysteine (Sec) is incorporated into a small group of proteins that are involved in preventing cellular oxidative stress. The Sec-specific elongation factor eEFec delivers the aminoacylated selenocysteine-tRNA (Sec-tRNASec) to the ribosome and suppresses UGA codons that are upstream of Sec Insertion Sequence (SECIS) elements bound by SECIS binding protein 2 (SBP2)[PMID:22992746]. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon.

Protocols

Product Specific Protocols
WB protocol for EEFSEC antibody 10628-1-APDownload protocol
IHC protocol for EEFSEC antibody 10628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Physiol Cell Physiol

Daily protein supplementation attenuates immobilization-induced blunting of postabsorptive muscle mTORC1 activation in middle-aged men.

Authors - Nina Zeng
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Linda (Verified Customer) (08-07-2023)

The antibody worked well in the experiment and I was able to detect the protein of interest

  • Applications: Western Blot
  • Cell Tissue Type: HEK293T