Validation Data Gallery
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Tested Applications
| Positive WB detected in | HT-29 cells, K-562 cells, mouse kidney tissue, JAR cells, SW480 cells, human heart tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human heart tissue, human prostate cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
12004-1-AP targets EFEMP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EFEMP2 fusion protein Ag2640 相同性解析による交差性が予測される生物種 |
| Full Name | EGF-containing fibulin-like extracellular matrix protein 2 |
| Calculated molecular weight | 443 aa, 49 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC010456 |
| Gene Symbol | EFEMP2 |
| Gene ID (NCBI) | 30008 |
| RRID | AB_10667002 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95967 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EFEMP2, also known as Fibulin-4 is a 60 kDa extracellular matrix (ECM) glycoprotein that is a pivotal oncogene in tumor progression and metastasis. EFEMP2 is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
| IHC protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
| IP protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Biol Ther STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2 | ||
Cell Mol Biol Lett EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells |