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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EFHD2 Polyclonal antibody (31624-1-AP)

    WB analysis using 31624-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31624-1-AP (EFHD2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EFHD2 Polyclonal antibody (31624-1-AP)

    IHC staining of mouse brain using 31624-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31624-1-AP (EFHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EFHD2 Polyclonal antibody (31624-1-AP)

    IHC staining of mouse brain using 31624-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31624-1-AP (EFHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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EFHD2 Polyclonal antibody

EFHD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31624-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, NCI-H1299 cells, SW480 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31624-1-AP targets EFHD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EFHD2 fusion protein Ag35084 相同性解析による交差性が予測される生物種
Full Name EF-hand domain family, member D2
Calculated molecular weight27 kDa
Observed molecular weight27-30 kDa
GenBank accession numberBC023611
Gene Symbol EFHD2
Gene ID (NCBI) 79180
RRIDAB_3670053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96C19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EF-hand domain-containing protein D2 (also known as Swiprosin-1) is a calcium-binding protein with two conserved EF-chiral coiled-coil structural domains, and it is mostly involved in calcium signaling, cytoskeleton assembly and synapse formation. EFHD2 is broadly expressed in various cell types, with particularly high expression in neurons.

Protocols

Product Specific Protocols
WB protocol for EFHD2 antibody 31624-1-APDownload protocol
IHC protocol for EFHD2 antibody 31624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols