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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EFHD2 Recombinant antibody, PBS Only

EFHD2 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240185H5

Cat no : 83264-5-PBS

Synonyms

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185H5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EFHD2 Recombinant antibody (83264-5-RR)

    WB analysis using 83264-5-RR (same clone as 83264-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83264-5-RR (EFHD2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EFHD2 Recombinant antibody (83264-5-RR)

    IF Staining of A431 using 83264-5-RR (same clone as 83264-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using EFHD2 antibody (83264-5-RR, Clone: 240185H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using EFHD2 Recombinant antibody (83264-5-RR)

    FC experiment of A431 using 83264-5-RR (same clone as 83264-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug EFHD2 Recombinant antibody (83264-5-RR, Clone:240185H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83264-5-RR

    Affinity and Kinetic Characterization of 83264-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83264-5-RR against Human EFHD2 were performed. The affinity constant is 1.42 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83264-5-PBS is EFHD2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EFHD2 fusion protein Ag35084 相同性解析による交差性が予測される生物種
Full Name EF-hand domain family, member D2
Calculated molecular weight27 kDa
GenBank accession numberBC023611
Gene symbol EFHD2
Gene ID (NCBI) 79180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.