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- KD/KO Validated
Ephrin A3 Polyclonal antibody
Ephrin A3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12480-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, A549 cells, rat brain tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IF | See 1 publications below |
Product Information
12480-1-AP targets Ephrin A3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Ephrin A3 fusion protein Ag3188 相同性解析による交差性が予測される生物種 |
| Full Name | ephrin-A3 |
| Calculated molecular weight | 238 aa, 26 kDa |
| Observed molecular weight | 26-35 kDa |
| GenBank accession number | BC017722 |
| Gene symbol | Ephrin A3 |
| Gene ID (NCBI) | 1944 |
| RRID | AB_10638305 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Ephrin A3 antibody 12480-1-AP | Download protocol |
| IHC protocol for Ephrin A3 antibody 12480-1-AP | Download protocol |
| IP protocol for Ephrin A3 antibody 12480-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Research (Wash D C) Exercise-Induced miR-210 Promotes Cardiomyocyte Proliferation and Survival and Mediates Exercise-Induced Cardiac Protection against Ischemia/Reperfusion Injury | ||
Cancer Med EphrinA3 is a key regulator of malignant behaviors and a potential prognostic factor in lung adenocarcinoma. | ||
Biomed Res Int OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway.
| ||
J Exp Clin Cancer Res The Ephrin tyrosine kinase a3 (EphA3) is a novel mediator of RAGE-prompted motility of breast cancer cells
| ||
Brain Res Bull Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats | ||
J Cell Mol Med microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3 |
