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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EFTUD1 Polyclonal antibody

EFTUD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24729-1-AP

Synonyms

EFTUD1, FAM42A, HsT19294, Protein FAM42A, RIA1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis of mouse brain using 24729-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis of fetal human brain using 24729-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    IHC staining of human kidney using 24729-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    IHC staining of human kidney using 24729-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24729-1-AP is EFTUD1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EFTUD1 fusion protein Ag20371 相同性解析による交差性が予測される生物種
Full Name elongation factor Tu GTP binding domain containing 1
Calculated molecular weight 1120 aa, 125 kDa
Observed molecular weight 125-135 kDa
GenBank accession numberBC156743
Gene symbol EFTUD1
Gene ID (NCBI) 79631
RRIDAB_2879693
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EFTUD1 antibody 24729-1-APDownload protocol
IHC protocol for EFTUD1 antibody 24729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols