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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EGFL7 Polyclonal antibody

EGFL7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26661-1-AP

Synonyms

Multiple EGF-like domains protein 7, MEGF7, Epidermal growth factor-like protein 7, EGF-like protein 7, EGF like protein 7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EGFL7 Polyclonal antibody (26661-1-AP)

    WB analysis using 26661-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26661-1-AP (EGFL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EGFL7 Polyclonal antibody (26661-1-AP)

    IHC staining of human placenta using 26661-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26661-1-AP (EGFL7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using EGFL7 Polyclonal antibody (26661-1-AP)

    IHC staining of human placenta using 26661-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26661-1-AP (EGFL7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inCOLO 320 cells, K-562 cells, RKO cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26661-1-AP targets EGFL7 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EGFL7 fusion protein Ag24693 相同性解析による交差性が予測される生物種
Full Name EGF-like-domain, multiple 7
Calculated molecular weight 273 aa, 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012377
Gene symbol EGFL7
Gene ID (NCBI) 51162
RRIDAB_3669560
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epidermal growth factor-like protein 7 (EGFL7, also known as MEGF7, ZNEU1, and VE-STATIN) is a proangiogenic factor that has been widely described as having a vital role in tubulogenesis and regulation of angiogenesis, mainly during embryogenesis organogenesis (PMID: 22160377; 15162510). It also can reduce central nervous system inflammation and may play a role in tumorigenesis (PMID: 29483510; 37490307).

Protocols

Product Specific Protocols
WB protocol for EGFL7 antibody 26661-1-APDownload protocol
IHC protocol for EGFL7 antibody 26661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols