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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Pikachurin Polyclonal antibody (14578-1-AP)

    WB analysis of human placenta using 14578-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14578-1-AP (Pikachurin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using Pikachurin Polyclonal antibody (14578-1-AP)

    WB analysis of PC-3 using 14578-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14578-1-AP (Pikachurin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using Pikachurin Polyclonal antibody (14578-1-AP)

    WB analysis of SH-SY5Y using 14578-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14578-1-AP (Pikachurin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using Pikachurin Polyclonal antibody (14578-1-AP)

    IF Staining of mouse eye using 14578-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Pikachurin antibody (14578-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Pikachurin Polyclonal antibody (14578-1-AP)

    IF Staining of SH-SY5Y using 14578-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Pikachurin antibody (14578-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Pikachurin Polyclonal antibody

Pikachurin Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 14578-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, CoIP, ELISA

Synonyms

EGFLAM, EGF-like, fibronectin type-III and laminin G-like domain-containing protein, AGRNL, Agrin-like protein, AGRINL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, PC-3 cells, SH-SY5Y cells
Positive IF-P detected inmouse eye tissue
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14578-1-AP targets Pikachurin in WB, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Pikachurin fusion protein Ag5986 相同性解析による交差性が予測される生物種
Full Name EGF-like, fibronectin type III and laminin G domains
Calculated molecular weight 111 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC033177
Gene Symbol Pikachurin
Gene ID (NCBI) 133584
RRIDAB_2277653
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ63HQ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This antibody detects a 60-kDa band, which has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

Protocols

Product Specific Protocols
WB protocol for Pikachurin antibody 14578-1-APDownload protocol
IF protocol for Pikachurin antibody 14578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Stem Cells Dev

Pikachurin Is Partially Involved in the Synaptic Connection Between Donor and Host Cells in Late-Stage rd1 Mice Following Conspecific Photoreceptor Transplantation.

Authors - Chen Liang
View Article
humanWB,CoIP

Int Immunopharmacol

EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma

Authors - Kaishu Li
View Article
mouseIF

iScience

The prion protein is required for normal responses to light stimuli by photoreceptors and bipolar cells

Authors - James F Striebel
View Article