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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EGFR Polyclonal antibody (30139-1-AP)

    WB analysis using 30139-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 30139-1-AP (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using EGFR Polyclonal antibody (30139-1-AP)

    WB analysis of A431 using 30139-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 30139-1-AP (EGFR antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using EGFR Polyclonal antibody (30139-1-AP)

    IP experiment of HeLa using 30139-1-AP

    IP result of anti-EGFR (IP:30139-1-AP, 4ug; Detection:30139-1-AP 1:4000) with HeLa cells lysate 1520 ug.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EGFR Polyclonal antibody (30139-1-AP)

    IF Staining of A431 using 30139-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR antibody (30139-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using EGFR Polyclonal antibody (30139-1-AP)

    IF Staining of HaCaT using 30139-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using EGFR antibody (30139-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using EGFR Polyclonal antibody (30139-1-AP)

    FC experiment of A431 using 30139-1-AP

    1x10^6 A431 cells were surface stained with 0.25 ug EGFR Polyclonal antibody (30139-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

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EGFR Polyclonal antibody

EGFR Polyclonal Antibody for WB, IF/ICC, FC, IP, ELISA
Cat No. 30139-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC, IP, ELISA

Synonyms

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inA431 cells, HaCaT cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30139-1-AP targets EGFR in WB, IF/ICC, FC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight134kd
Observed molecular weight 145-165 kDa
GenBank accession numberNM_005228.5
Gene Symbol EGFR
Gene ID (NCBI) 1956
RRIDAB_3086243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGFR is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer.

Protocols

Product Specific Protocols
WB protocol for EGFR antibody 30139-1-APDownload protocol
IF protocol for EGFR antibody 30139-1-APDownload protocol
IP protocol for EGFR antibody 30139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols