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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EGFR Monoclonal antibody

EGFR Monoclonal Antibody for WB, IF/ICC, FC, ELISA, Blocking

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC, ELISA, Blocking

Conjugate

Unconjugated

CloneNo.

2C8C12

Cat no : 68643-1-Ig

Synonyms

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 2C8C12

Validation Data Gallery

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  • Western Blot (WB) analysis of SCaBER cells using EGFR Monoclonal antibody (68643-1-Ig)

    WB analysis of SCaBER using 68643-1-Ig

    SCaBER cells were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-468 cells using EGFR Monoclonal antibody (68643-1-Ig)

    WB analysis of MDA-MB-468 using 68643-1-Ig

    MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EGFR Monoclonal antibody (68643-1-Ig)

    WB analysis using 68643-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using EGFR Monoclonal antibody (68643-1-Ig)

    IF Staining of HaCaT using 68643-1-Ig

    Immunofluorescent analysis of un-fixed HaCaT cells using EGFR antibody (68643-1-Ig, Clone: 2C8C12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EGFR Monoclonal antibody (68643-1-Ig)

    IF Staining of A431 using 68643-1-Ig

    Immunofluorescent analysis of un-fixed A431 cells using EGFR antibody (68643-1-Ig, Clone: 2C8C12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Flow cytometry (FC) experiment of A431 cells using EGFR Monoclonal antibody (68643-1-Ig)

    FC experiment of A431 using 68643-1-Ig

    1x10^6 A431 cells were surface stained with 0.2 μg EGFR Monoclonal Antibody (68643-1-Ig, Clone:2C8C12) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(red), or 0.2 μg Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were not fixed.

  • Blocking experiment of HeLa cells using EGFR Monoclonal antibody (68643-1-Ig)

    Blocking experiment of HeLa cells using 68643-1-Ig

    Human EGF (Cat.NO. HZ-1326 ) stimulates proliferation of HeLa cells in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human EGF (Cat.NO. HZ-1326 ) is blocked by mouse anti-human EGFR monoclonal antibody 68643-1-Ig at serial dose (red curve, refer to top X-right Y axis). The EC50 is typically 0.2-0.8 μg/mL at the presence of 1ng/ml EGF (Cat.NO. HZ-1326 ).

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Tested Applications

Positive WB detected inSCaBER cells, HaCaT cells, MDA-MB-468 cells, A431 cells
Positive IF/ICC detected inHaCaT cells, A431 cells
Positive FC detected inA431 cells
Positive Blocking detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
BLOCKINGBLOCKING : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68643-1-Ig targets EGFR in WB, IF/ICC, FC, ELISA, Blocking applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EGFR fusion protein Ag24947 相同性解析による交差性が予測される生物種
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight134kd
Observed molecular weight160 kDa
GenBank accession numberNM_005228.5
Gene symbol EGFR
Gene ID (NCBI) 1956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGFR, also named ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. When stained with live cells, EGFR can be transported into the cell interior, which is consistent with the literature report (PMID: 18084617).

Protocols

Product Specific Protocols
WB protocol for EGFR antibody 68643-1-IgDownload protocol
IF protocol for EGFR antibody 68643-1-IgDownload protocol
FC protocol for EGFR antibody 68643-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols