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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EGFR (C-terminal) Polyclonal antibody, PBS Only

EGFR (C-terminal) Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51071-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using EGFR (C-terminal) Polyclonal antibody (51071-2-AP)

    WB analysis of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using EGFR (C-terminal) Polyclonal antibody (51071-2-AP)

    WB analysis of L02 using 51071-2-AP (same clone as 51071-2-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EGFR (C-terminal) Polyclonal antibody (51071-2-AP)

    WB analysis of HeLa using 51071-2-AP (same clone as 51071-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A431 cells using EGFR (C-terminal) Polyclonal antibody (51071-2-AP)

    IP experiment of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    IP result of anti-EGFR (C-terminal) (IP:51071-2-AP, 4ug; Detection:51071-2-AP 1:30000) with A431 cells lysate 1200 ug. This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EGFR (C-terminal) Polyclonal antibody (51071-2-AP)

    IF Staining of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR (C-terminal) antibody (51071-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51071-2-PBS targets EGFR (C-terminal) in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight 1210 aa, 134 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC094761
Gene symbol EGFR
Gene ID (NCBI) 1956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. The antibody is specific to isoform1.