Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

WB result of anti-EGFR (18986-1-AP) in different cell lysates.

WB result of EGFR-Specific antibody (18986-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR-Specific transfected HepG2 cells.

WB result ofanti-EGFR (18986-1-AP) in Hela and REP1 cell by Dr.Kodani, Andrew.

NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

HeLa cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

L02 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

MCF7 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

A431 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18986-1-AP (EGFR-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18986-1-AP (EGFR-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-EGFR-Specific (IP:18986-1-AP, 3ug; Detection:18986-1-AP 1:500) with MCF-7 cells lysate 2500ug.

Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EGFR-Specific antibody (18986-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 A431 cells were stained with 0.2 ug Anti-Human EGFR-Specific (18986-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

1X10^6 A431 cells were stained with .2ug EGFR-Specific antibody (18986-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.