Validation Data Gallery

Filter: AllWBIP

WB analysis using 18986-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18986-1-AP
WB result of EGFR-Specific antibody (18986-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR-Specific transfected HepG2 cells.
WB analysis of HeLa/RPE1 cells using 18986-1-AP
WB result ofanti-EGFR (18986-1-AP) in Hela and REP1 cell by Dr.Kodani, Andrew.
WB analysis using 18986-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 18986-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of NCI-H1299 using 18986-1-AP
NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 18986-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
WB analysis of MCF-7 using 18986-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 18986-1-AP
IP result of anti-EGFR-Specific (IP:18986-1-AP, 3ug; Detection:18986-1-AP 1:500) with MCF-7 cells lysate 2500ug.

Featured Product
KD/KO Validated

EGFR-Specific Polyclonal antibody

EGFR-Specific Polyclonal Antibody for WB, IP, ELISA

Cat No. 18986-1-AP

Publications(165)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Synonyms

EGFR, EGFR-Specific, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (165)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, MCF-7 cells, A431 cells, HeLa/RPE1 cells, NCI-H1299 cells, HeLa cells, A549 cells, L02 cells, NCL-H1299 cells
Positive IP detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 140 publications below
IHC	See 23 publications below
IF	See 21 publications below
IP	See 9 publications below
FC	See 1 publications below
CoIP	See 6 publications below
ChIP	See 1 publications below

Product Information

18986-1-AP targets EGFR-Specific in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, pig, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight	134 kDa
Observed molecular weight	145-165 kDa
GenBank accession number	NM_005228
Gene Symbol	EGFR
Gene ID (NCBI)	1956
RRID	AB_10596476
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P00533
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.

Protocols

Product Specific Protocols
WB protocol for EGFR-Specific antibody 18986-1-AP	Download protocol
IP protocol for EGFR-Specific antibody 18986-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Nat Microbiol Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells Authors - Yinggui Yang View Article
mouse	WB,IHC	Cell Res In vivo self-assembled small RNAs as a new generation of RNAi therapeutics. Authors - Zheng Fu KD Validated View Article
human	WB	Cancer Commun (Lond) Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma Authors - Xiaoteng Cui View Article
human	IF	ACS Appl Mater Interfaces On-Chip Replication of Extremely Early-Stage Tumor Behavior. Authors - Chengpan Li View Article
pig	WB	Biosens Bioelectron Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic. Authors - Yi-Jyun Chen View Article
human	WB, IHC, ChIP	Cancer Res Hypoxia Alters the Response to Anti-EGFR Therapy by Regulating EGFR Expression and Downstream Signaling in a DNA Methylation-Specific and HIF-Dependent Manner. Authors - Mahelet Mamo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18986-1-AP

WB analysis of HepG2 using 18986-1-AP

WB analysis of HeLa/RPE1 cells using 18986-1-AP

WB analysis using 18986-1-AP

WB analysis using 18986-1-AP

WB analysis of NCI-H1299 using 18986-1-AP

WB analysis of A431 using 18986-1-AP

WB analysis of MCF-7 using 18986-1-AP

IP experiment of MCF-7 using 18986-1-AP

EGFR-Specific Polyclonal antibody

EGFR-Specific Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma

On-Chip Replication of Extremely Early-Stage Tumor Behavior.

Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic.

Hypoxia Alters the Response to Anti-EGFR Therapy by Regulating EGFR Expression and Downstream Signaling in a DNA Methylation-Specific and HIF-Dependent Manner.