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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EGLN3/PHD3 Polyclonal antibody

EGLN3/PHD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27875-1-AP

Synonyms

EGLN3, PHD3, HIF prolyl hydroxylase 3, HIF PH3, Egl nine homolog 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EGLN3/PHD3 Polyclonal antibody (27875-1-AP)

    WB analysis using 27875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EGLN3/PHD3 Polyclonal antibody (27875-1-AP)

    IHC staining of mouse colon using 27875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EGLN3/PHD3 Polyclonal antibody (27875-1-AP)

    IHC staining of mouse colon using 27875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27875-1-AP targets EGLN3/PHD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EGLN3/PHD3 fusion protein Ag27464 相同性解析による交差性が予測される生物種
Full Name egl nine homolog 3 (C. elegans)
Calculated molecular weight 27 kDa
Observed molecular weight27 kDa
GenBank accession numberBC010992
Gene symbol EGLN3
Gene ID (NCBI) 112399
RRIDAB_3086001
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

Protocols

Product Specific Protocols
WB protocol for EGLN3/PHD3 antibody 27875-1-APDownload protocol
IHC protocol for EGLN3/PHD3 antibody 27875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols