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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EGLN3/PHD3 Polyclonal antibody

EGLN3/PHD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27875-1-AP

Synonyms

EGLN3, PHD3, HIF prolyl hydroxylase 3, HIF PH3, Egl nine homolog 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EGLN3/PHD3 Polyclonal antibody (27875-1-AP)

    WB analysis using 27875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EGLN3/PHD3 Polyclonal antibody (27875-1-AP)

    IHC staining of mouse colon using 27875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EGLN3/PHD3 Polyclonal antibody (27875-1-AP)

    IHC staining of mouse colon using 27875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27875-1-AP targets EGLN3/PHD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EGLN3/PHD3 fusion protein Ag27464 相同性解析による交差性が予測される生物種
Full Name egl nine homolog 3 (C. elegans)
Calculated molecular weight 27 kDa
Observed molecular weight27 kDa
GenBank accession numberBC010992
Gene symbol EGLN3
Gene ID (NCBI) 112399
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

Protocols

Product Specific Protocols
WB protocol for EGLN3/PHD3 antibody 27875-1-APDownload protocol
IHC protocol for EGLN3/PHD3 antibody 27875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols