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KD/KO Validated

EGR1 Polyclonal antibody

Name: EGR1 Polyclonal antibody
Brand: Proteintech
SKU: 22008-1-AP

EGR1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22008-1-AP

Synonyms

G0S30, EGR-1, EGR 1, Early growth response protein 1, early growth response 1

Validation Data Gallery

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WB analysis using 22008-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22008-1-AP (EGR1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 22008-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22008-1-AP (EGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 22008-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22008-1-AP (EGR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 22008-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22008-1-AP (EGR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 22008-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22008-1-AP (EGR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 22008-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22008-1-AP (EGR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 22008-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, RAW264.7, Jurkat cells, Ramos cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 37 publications below
IHC	See 9 publications below
IF	See 9 publications below
IP	See 1 publications below
ELISA	See 1 publications below
ChIP	See 3 publications below

Product Information

22008-1-AP targets EGR1 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EGR1 fusion protein Ag13395 相同性解析による交差性が予測される生物種
Full Name	early growth response 1
Calculated molecular weight	543 aa, 58 kDa
Observed molecular weight	70-80 kDa
GenBank accession number	BC073983
Gene symbol	EGR1
Gene ID (NCBI)	1958
RRID	AB_11182923
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EGR1, also named as Early growth response protein 1, is a 543 amino acid protein, which belongs to the EGR C2H2-type zinc-finger protein family. EGR1 as a transcriptional regulator recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes and binds double-stranded target DNA, irrespective of the cytosine methylation status. EGR1 regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. EGR1 activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation and plays a role in the regulation of cell survival, proliferation and cell death. Egr1 is suggested to be a 55-kDa protein according to the translation of its coding sequence. Analysis of cytoplasmic and nuclear fractions of normal and irradiated native CNS tissue by Western blotting revealed a 110-kDa band for Egr1 localized in the cytoplasm and a 75-kDa version for the nuclear Egr1(PMID: 17497096).

Protocols

Product Specific Protocols
WB protocol for EGR1 antibody 22008-1-AP	Download protocol
IHC protocol for EGR1 antibody 22008-1-AP	Download protocol
IF protocol for EGR1 antibody 22008-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Dev Cell PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine. Authors - Deguan Lv KD Validated View Article
mouse	WB	Mol Ther Nucleic Acids Genetic or siRNA inhibition of MBD2 attenuates the UUO- and I/R-induced renal fibrosis via downregulation of EGR1. Authors - Kai Ai KD Validated View Article
human	WB	Pharmacol Res Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin. Authors - Long Wang View Article
mouse	WB,ChIP	Inflammation PD-L1 Expression Is Increased in LPS-Induced Acute Respiratory Distress Syndrome by PI3K-AKT-Egr-1/C/EBPδ Signaling Pathway Authors - Chunguang Yan View Article
human	WB	J Cell Mol Med EGR1 promoted anticancer effects of Scutellarin via regulating LINC00857/miR-150-5p/c-Myc in osteosarcoma. Authors - Jian Han KD Validated View Article
rat	IF	Phytomedicine Stigmasterol upregulates immediate early genes and promotes neuronal cytoarchitecture in primary hippocampal neurons as revealed by transcriptome analysis. Authors - Md Nazmul Haque View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EGR1 Polyclonal antibody

EGR1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Validation Data Gallery

WB analysis using 22008-1-AP

WB analysis using 22008-1-AP

WB analysis of RAW264.7 using 22008-1-AP

IHC staining of human ovary tumor using 22008-1-AP

IHC staining of human ovary tumor using 22008-1-AP

IF Staining of mouse brain using 22008-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.

Genetic or siRNA inhibition of MBD2 attenuates the UUO- and I/R-induced renal fibrosis via downregulation of EGR1.

Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin.

PD-L1 Expression Is Increased in LPS-Induced Acute Respiratory Distress Syndrome by PI3K-AKT-Egr-1/C/EBPδ Signaling Pathway

EGR1 promoted anticancer effects of Scutellarin via regulating LINC00857/miR-150-5p/c-Myc in osteosarcoma.

Stigmasterol upregulates immediate early genes and promotes neuronal cytoarchitecture in primary hippocampal neurons as revealed by transcriptome analysis.