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  • KD/KO Validated

EHBP1 Polyclonal antibody

EHBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17637-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

NACSIN, HPC12, EH domain-binding protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inDU 145 cells, HepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman testis tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17637-1-AP targets EHBP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EHBP1 fusion protein Ag11916 相同性解析による交差性が予測される生物種
Full Name EH domain binding protein 1
Calculated molecular weight132 kDa
Observed molecular weight 160-180 kDa
GenBank accession numberBC067215
Gene Symbol EHBP1
Gene ID (NCBI) 23301
RRIDAB_2097216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NDI1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EHBP1 antibody 17637-1-APDownload protocol
IHC protocol for EHBP1 antibody 17637-1-APDownload protocol
IF protocol for EHBP1 antibody 17637-1-APDownload protocol
IP protocol for EHBP1 antibody 17637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

LRRK2 and its substrate Rab GTPases are sequentially targeted onto stressed lysosomes and maintain their homeostasis.

Authors - Tomoya Eguchi
  • KD Validated
humanWB

Sci Adv

A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets.

Authors - Zhipeng Li
  • KD Validated
humanIF

Mol Cell

The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.

Authors - Joanna R Kovalski
mouseWB,IF

Nat Commun

The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane

Authors - Claudia Matthaeus
humanWB

J Cell Biol

VPS13C regulates phospho-Rab10-mediated lysosomal function in human dopaminergic neurons

Authors - Leonie F Schrӧder
mouse,ratWB,IP,CoIP

J Neurosci

EHBP1 Is Critically Involved in the Dendritic Arbor Formation and Is Coupled to Factors Promoting Actin Filament Formation

Authors - Yuanyuan Ji
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