Validation Data Gallery
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Tested Applications
| Positive WB detected in | DU 145 cells, HepG2 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human testis tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
17637-1-AP targets EHBP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EHBP1 fusion protein Ag11916 相同性解析による交差性が予測される生物種 |
| Full Name | EH domain binding protein 1 |
| Calculated molecular weight | 132 kDa |
| Observed molecular weight | 160-180 kDa |
| GenBank accession number | BC067215 |
| Gene Symbol | EHBP1 |
| Gene ID (NCBI) | 23301 |
| RRID | AB_2097216 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NDI1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EHBP1 antibody 17637-1-AP | Download protocol |
| IHC protocol for EHBP1 antibody 17637-1-AP | Download protocol |
| IF protocol for EHBP1 antibody 17637-1-AP | Download protocol |
| IP protocol for EHBP1 antibody 17637-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A LRRK2 and its substrate Rab GTPases are sequentially targeted onto stressed lysosomes and maintain their homeostasis.
| ||
Sci Adv A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets.
| ||
Nat Commun The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane | ||
J Cell Biol VPS13C regulates phospho-Rab10-mediated lysosomal function in human dopaminergic neurons | ||
J Neurosci EHBP1 Is Critically Involved in the Dendritic Arbor Formation and Is Coupled to Factors Promoting Actin Filament Formation |