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KD/KO Validated

EHD2 Polyclonal antibody

Name: EHD2 Polyclonal antibody
Brand: Proteintech
SKU: 11440-1-AP
Rating: 5.00 (2 reviews)

EHD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 11440-1-AP

Synonyms

PAST2, PAST homolog 2, EH domain-containing protein 2, EH domain containing protein 2, EH domain containing 2

Validation Data Gallery

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AllWBIPIHC

WB analysis using 11440-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11440-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 11440-1-AP
IP result of anti-EHD2 (IP:11440-1-AP, 4ug; Detection:11440-1-AP 1:800) with mouse heart tissue lysate 4000ug.
IHC staining of human breast cancer using 11440-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11440-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 11440-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 11440-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 11440-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 11440-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 11440-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 11440-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 11440-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human heart tissue, mouse heart tissue, rat heart tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human breast cancer tissue, human normal colon, human oesophagus cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

11440-1-AP targets EHD2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EHD2 fusion protein Ag2002 相同性解析による交差性が予測される生物種
Full Name	EH-domain containing 2
Calculated molecular weight	543 aa, 61 kDa
Observed molecular weight	61 kDa
GenBank accession number	BC014445
Gene symbol	EHD2
Gene ID (NCBI)	30846
RRID	AB_2097325
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for EHD2 antibody 11440-1-AP	Download protocol
IHC protocol for EHD2 antibody 11440-1-AP	Download protocol
IP protocol for EHD2 antibody 11440-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Am J Cancer Res Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B. Authors - Junqiang Li KD Validated View Article
human	IF	PLoS One Super-resolution analysis of PACSIN2 and EHD2 at caveolae. Authors - Tamako Nishimura View Article
human	IHC	Cancer Invest EHD2 Overexpression Suppresses the Proliferation, Migration, and Invasion in Human Colon Cancer. Authors - Chengqi Guan View Article
	WB	Cancer Invest Identification of Differentially Expressed Proteins of Normal and Cancerous Human Colorectal Tissues by Liquid Chromatograph-Mass Spectrometer Based on iTRAQ Approach. Authors - Jingjing Lv View Article
mouse	WB	Nature Post-translational control of beige fat biogenesis by PRDM16 stabilization Authors - Qiang Wang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Laura (Verified Customer) (01-27-2022)	It works well for western blot with different bands but one specific around 65 kDa. It also works well for immunofluorescence of cells. Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1/1000WB 1/100IF Cell Tissue Type: MOVAS (Mouse Primary vascular aortic smooth muscle cells) and RPE-1 (Human retinal pigment epithelial-1)
FH Kyosuke (Verified Customer) (06-12-2019)	It works well on HUVECs sample for Western blot. Applications: Western Blot, Primary Antibody Dilution: 1/1000 Cell Tissue Type: HUVECs

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EHD2 Polyclonal antibody

EHD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 11440-1-AP

WB analysis of human heart using 11440-1-AP

IP experiment of mouse heart using 11440-1-AP

IHC staining of human breast cancer using 11440-1-AP

IHC staining of human breast cancer using 11440-1-AP

IHC staining of human normal colon using 11440-1-AP

IHC staining of human normal colon using 11440-1-AP

IHC staining of human oesophagus cancer using 11440-1-AP

IHC staining of human oesophagus cancer using 11440-1-AP

IHC staining of human ovary tumor using 11440-1-AP

IHC staining of human ovary tumor using 11440-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B.

Super-resolution analysis of PACSIN2 and EHD2 at caveolae.

EHD2 Overexpression Suppresses the Proliferation, Migration, and Invasion in Human Colon Cancer.

Identification of Differentially Expressed Proteins of Normal and Cancerous Human Colorectal Tissues by Liquid Chromatograph-Mass Spectrometer Based on iTRAQ Approach.

Post-translational control of beige fat biogenesis by PRDM16 stabilization

Reviews