Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, human heart tissue, mouse heart tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human breast cancer tissue, human normal colon, human oesophagus cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
11440-1-AP targets EHD2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EHD2 fusion protein Ag2002 相同性解析による交差性が予測される生物種 |
| Full Name | EH-domain containing 2 |
| Calculated molecular weight | 543 aa, 61 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | BC014445 |
| Gene Symbol | EHD2 |
| Gene ID (NCBI) | 30846 |
| RRID | AB_2097325 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZN4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EH domain-containing protein 2 (EHD2) is a member of the EHD protein family. It is primarily located in the plasma membrane but can also be found in the cytoplasm and endosomes. EHD2 is also a nuclear-cytoplasmic shuttle protein, acting as a corepressor of transcription to inhibit gene transcription when it enters the cell nucleus. EHD2 is a dynamin-related ATPase that plays a key role in regulating a series of biological processes. As a crucial regulator of endocytic transport, it is involved in the formation and maintenance of endosomal tubules and vesicles, which are vital for intracellular transport of proteins and other substances. EHD2 has been implicated in several human cancers and has potential as a predictive biomarker of chemotherapy efficacy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EHD2 antibody 11440-1-AP | Download protocol |
| IHC protocol for EHD2 antibody 11440-1-AP | Download protocol |
| IP protocol for EHD2 antibody 11440-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Cancer Res Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B.
| ||
Cancer Invest EHD2 Overexpression Suppresses the Proliferation, Migration, and Invasion in Human Colon Cancer. | ||
Cancer Invest Identification of Differentially Expressed Proteins of Normal and Cancerous Human Colorectal Tissues by Liquid Chromatograph-Mass Spectrometer Based on iTRAQ Approach. | ||
Emerg Microbes Infect Identification of cepharanthine as an effective inhibitor of African swine fever virus replication
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