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KD/KO Validated

EHD3 Polyclonal antibody

Name: EHD3 Polyclonal antibody
Brand: Proteintech
SKU: 25320-1-AP
Rating: 5.00 (1 reviews)

EHD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 25320-1-AP

Synonyms

EH domain containing 3, EH domain containing protein 3, EHD2, EHD3, PAST homolog 3, PAST3

Validation Data Gallery

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AllWBIHC

WB analysis using 25320-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human peripheral blood platelets using 25320-1-AP
human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 25320-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 25320-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of mouse lung using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25320-1-AP (EHD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25320-1-AP (EHD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 25320-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, human peripheral blood platelets, rat brain tissue, mouse kidney tissue
Positive IHC detected in	mouse lung tissue, human brain tissue, mouse brain tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

The immunogen of 25320-1-AP is EHD3 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EHD3 fusion protein Ag18216 相同性解析による交差性が予測される生物種
Full Name	EH-domain containing 3
Calculated molecular weight	535 aa, 61 kDa
Observed molecular weight	61 kDa
GenBank accession number	BC156996
Gene symbol	EHD3
Gene ID (NCBI)	30845
RRID	AB_2880029
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for EHD3 antibody 25320-1-AP	Download protocol
IHC protocol for EHD3 antibody 25320-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
zebrafish	WB	Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Authors - Quanlong Lu KD Validated View Article
mouse, zebrafish	WB,IF	Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Authors - Quanlong Lu KD Validated View Article
human	WB	EBioMedicine EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure. Authors - Quan Zhou View Article
human	WB	EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome. Authors - Tatsuo Miyamoto KO Validated View Article
rat	IHC	Int J Mol Sci Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases Authors - Mar Gil View Article
mouse	IHC	Am J Physiol Renal Physiol Unique miRNome and transcriptome profiles underlie microvascular heterogeneity in mouse kidney Authors - Matthijs Luxen View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Kyosuke (Verified Customer) (06-12-2019)

I works well on HUVECs for Western blot.

Applications: Western Blot,
Primary Antibody Dilution: 1/1000
Cell Tissue Type: HUVECs

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EHD3 Polyclonal antibody

EHD3 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 25320-1-AP

WB analysis of human peripheral blood platelets using 25320-1-AP

WB analysis of rat brain using 25320-1-AP

WB analysis of mouse kidney using 25320-1-AP

IHC staining of mouse lung using 25320-1-AP

IHC staining of mouse lung using 25320-1-AP

IHC staining of mouse brain using 25320-1-AP

IHC staining of mouse brain using 25320-1-AP

IHC staining of human brain using 25320-1-AP

IHC staining of human brain using 25320-1-AP

IHC staining of mouse kidney using 25320-1-AP

IHC staining of mouse kidney using 25320-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure.

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases

Unique miRNome and transcriptome profiles underlie microvascular heterogeneity in mouse kidney

Reviews