Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, mouse testis tissue, HeLa cells, HEK-293T cells, HepG2 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
11382-2-AP targets EHD4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EHD4 fusion protein Ag1942 相同性解析による交差性が予測される生物種 |
| Full Name | EH-domain containing 4 |
| Calculated molecular weight | 541 aa, 61 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | BC006287 |
| Gene Symbol | EHD4 |
| Gene ID (NCBI) | 30844 |
| RRID | AB_2277764 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H223 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
H-domain containing 4, also known as EHD4, belongs to the EHD protein family. Northern blot analysis detected expression of 4 mRNA species of approximately 2.0, 2.7, 3.2, and 3.6 kb. All of the transcripts are highly expressed in pancreas, and the 2.7- and 3.6-kb transcripts are highly expressed in heart. Only weak expression is found in other tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EHD4 antibody 11382-2-AP | Download protocol |
| IHC protocol for EHD4 antibody 11382-2-AP | Download protocol |
| IF protocol for EHD4 antibody 11382-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure. | ||
J Am Soc Nephrol Reduced Glomerular Filtration in Diabetes is Attributable to Loss of Density and Increased Resistance of Glomerular Endothelial Cell Fenestrations. | ||
Curr Biol EHD Proteins Cooperate to Generate Caveolar Clusters and to Maintain Caveolae during Repeated Mechanical Stress. | ||
Life Sci EHD3 positively regulated by NR5A1 participates in testosterone synthesis via endocytosis. |