Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 26570-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of SMMC-7721 using 26570-1-AP
SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
WB analysis of HepG2 using 26570-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 26570-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 26570-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26570-1-AP (EHHADH Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 26570-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26570-1-AP (EHHADH Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 26570-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 26570-1-AP (EHHADH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

EHHADH Polyclonal antibody

EHHADH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26570-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ECHD, EHHADH, L PBE, LBFP, LBP, PBE, PBFE

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, SMMC-7721 cells, HepG2 cells, mouse liver tissue, rat kidney tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below

Product Information

26570-1-AP targets EHHADH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EHHADH fusion protein Ag24237 相同性解析による交差性が予測される生物種
Full Name	enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
Calculated molecular weight	723 aa, 79 kDa
Observed molecular weight	69 kDa
GenBank accession number	BC038948
Gene Symbol	EHHADH
Gene ID (NCBI)	1962
RRID	AB_2880556
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q08426
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for EHHADH antibody 26570-1-AP	Download protocol
IHC protocol for EHHADH antibody 26570-1-AP	Download protocol
IF protocol for EHHADH antibody 26570-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation Authors - Yunzi Mao View Article
mouse	WB	Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity Authors - Zilong Zhang View Article
mouse	WB	Cell Rep Feedback repression of PPARα signaling by Let-7 microRNA. Authors - Tomoki Yagai View Article
mouse	WB	Int J Mol Sci Ameliorative Effect of Ethanolic Echinacea purpurea against Hyperthyroidism-Induced Oxidative Stress via AMRK and PPAR Signal Pathway Using Transcriptomics and Network Pharmacology Analysis Authors - Yingli Zhu View Article
mouse	WB	Int J Biochem Cell Biol Tamoxifen upregulates the peroxisomal β-oxidation enzyme Enoyl CoA hydratase and 3-hydroxyacyl CoA hydratase ameliorating hepatic lipid accumulation in mice Authors - Ziling Zhang View Article
mouse	WB	Geroscience Coordinated transcriptional upregulation of oxidative metabolism proteins in long-lived endocrine mutant mice Authors - Ahmed M Elmansi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26570-1-AP

WB analysis of SMMC-7721 using 26570-1-AP

WB analysis of HepG2 using 26570-1-AP

WB analysis of mouse liver using 26570-1-AP

IHC staining of human liver cancer using 26570-1-AP

IHC staining of human liver cancer using 26570-1-AP

IF Staining of HepG2 using 26570-1-AP

EHHADH Polyclonal antibody

EHHADH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity

Feedback repression of PPARα signaling by Let-7 microRNA.

Ameliorative Effect of Ethanolic Echinacea purpurea against Hyperthyroidism-Induced Oxidative Stress via AMRK and PPAR Signal Pathway Using Transcriptomics and Network Pharmacology Analysis

Tamoxifen upregulates the peroxisomal β-oxidation enzyme Enoyl CoA hydratase and 3-hydroxyacyl CoA hydratase ameliorating hepatic lipid accumulation in mice

Coordinated transcriptional upregulation of oxidative metabolism proteins in long-lived endocrine mutant mice