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KD/KO Validated

EHMT2 Polyclonal antibody

Name: EHMT2 Polyclonal antibody
Brand: Proteintech
SKU: 29303-1-AP

EHMT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 29303-1-AP

Synonyms

BAT8, C6orf30, DKFZp686H08213, EHMT2, FLJ35547, G9A, H3 K9 HMTase 3, HLA B associated transcript 8, KMT1C, Lysine N methyltransferase 1C, NG36, NG36/G9a, Protein G9a

Validation Data Gallery

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AllWBIHC

WB analysis using 29303-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29303-1-AP (EHMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 29303-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29303-1-AP (EHMT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 29303-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29303-1-AP (EHMT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 29303-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 29303-1-AP (EHMT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 29303-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, NIH/3T3 cells
Positive IHC detected in	human lung cancer tissue, human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 2 publications below
IHC	See 2 publications below
IP	See 1 publications below

Product Information

29303-1-AP targets EHMT2 in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity	Human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EHMT2 fusion protein Ag29160 相同性解析による交差性が予測される生物種
Full Name	euchromatic histone-lysine N-methyltransferase 2
Calculated molecular weight	1210 aa, 132 kDa
Observed molecular weight	160-180 kDa
GenBank accession number	BC018718
Gene symbol	EHMT2
Gene ID (NCBI)	10919
RRID	AB_2918275
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for EHMT2 antibody 29303-1-AP	Download protocol
IHC protocol for EHMT2 antibody 29303-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Nucleic Acids Res ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication Authors - Huanzhou Xu View Article
human	WB,IP	Oncogenesis Calreticulin enhances gastric cancer metastasis by dimethylating H3K9 in the E-cadherin promoter region mediating by G9a. Authors - Lina Wang KD Validated View Article
human	IHC	Mol Biotechnol Inhibition of the Tumor Suppressor Gene SPINK5 via EHMT2 Induces the Oral Squamous Cell Carcinoma Development Authors - Suzhen Sun KD Validated View Article
human	WB,IHC	Mol Carcinog A novel high-risk model identified by epithelial-mesenchymal transition predicts prognosis and radioresistance in rectal cancer Authors - Feiyu Qin View Article

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研究分野

Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

EHMT2 Polyclonal antibody

EHMT2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 29303-1-AP

IHC staining of human lung cancer using 29303-1-AP

IHC staining of human lung cancer using 29303-1-AP

IHC staining of human urothelial carcinoma using 29303-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication

Calreticulin enhances gastric cancer metastasis by dimethylating H3K9 in the E-cadherin promoter region mediating by G9a.

Inhibition of the Tumor Suppressor Gene SPINK5 via EHMT2 Induces the Oral Squamous Cell Carcinoma Development

A novel high-risk model identified by epithelial-mesenchymal transition predicts prognosis and radioresistance in rectal cancer