• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

EHMT2 Monoclonal antibody, PBS Only

EHMT2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12E3

Cat no : 66689-1-PBS

Synonyms

EHMT2/G9a, Euchromatic histone-lysine N-methyltransferase 2, EC:2.1.1.-, C6orf30, BAT8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using EHMT2 Monoclonal antibody (66689-1-Ig)

    WB analysis of HeLa using 66689-1-Ig (same clone as 66689-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EHMT2 Monoclonal antibody (66689-1-Ig)

    WB analysis of HEK-293 using 66689-1-Ig (same clone as 66689-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EHMT2 Monoclonal antibody (66689-1-Ig)

    WB analysis of HEK-293 using 66689-1-Ig (same clone as 66689-1-PBS)

    WB result of EHMT2 antibody (66689-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EHMT2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EHMT2 Monoclonal antibody (66689-1-Ig)

    IF Staining of A431 using 66689-1-Ig (same clone as 66689-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EHMT2 antibody (66689-1-Ig, Clone: 1A12E3 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

☆1/31（金）までお得に買える！ >> IHC・ELISA・IPキット最大30%OFFキャンペーン

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66689-1-PBS targets EHMT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EHMT2 fusion protein Ag27477 相同性解析による交差性が予測される生物種
Full Name euchromatic histone-lysine N-methyltransferase 2
Calculated molecular weight 1210 aa, 132 kDa
Observed molecular weight 150 kDa and 170 kDa
GenBank accession numberBC018718
Gene symbol EHMT2
Gene ID (NCBI) 10919
RRIDAB_2882043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.