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  • Featured Product
  • KD/KO Validated

EHMT2 Monoclonal antibody, PBS Only

EHMT2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12E3

Cat no : 66689-1-PBS

Synonyms

EHMT2/G9a, Euchromatic histone-lysine N-methyltransferase 2, EC:2.1.1.-, C6orf30, BAT8

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EHMT2 Monoclonal antibody (66689-1-Ig)

    WB analysis of HeLa using 66689-1-Ig (same clone as 66689-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EHMT2 Monoclonal antibody (66689-1-Ig)

    WB analysis of HEK-293 using 66689-1-Ig (same clone as 66689-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EHMT2 Monoclonal antibody (66689-1-Ig)

    WB analysis of HEK-293 using 66689-1-Ig (same clone as 66689-1-PBS)

    WB result of EHMT2 antibody (66689-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EHMT2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EHMT2 Monoclonal antibody (66689-1-Ig)

    IF Staining of A431 using 66689-1-Ig (same clone as 66689-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EHMT2 antibody (66689-1-Ig, Clone: 1A12E3 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66689-1-PBS targets EHMT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EHMT2 fusion protein Ag27477 相同性解析による交差性が予測される生物種
Full Name euchromatic histone-lysine N-methyltransferase 2
Calculated molecular weight 1210 aa, 132 kDa
Observed molecular weight 150 kDa and 170 kDa
GenBank accession numberBC018718
Gene symbol EHMT2
Gene ID (NCBI) 10919
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.