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  • Featured Product
  • KD/KO Validated

EI24 Polyclonal antibody

EI24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20456-1-AP

Synonyms

EI24, etoposide induced 2.4 mRNA, p53 induced gene 8 protein, p53-induced factor Ei24, PIG8, TP53I8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EI24 Polyclonal antibody (20456-1-AP)

    WB analysis using 20456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20456-1-AP (EI24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EI24 Polyclonal antibody (20456-1-AP)

    IHC staining of human colon cancer using 20456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20456-1-AP (EI24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EI24 Polyclonal antibody (20456-1-AP)

    IHC staining of human colon cancer using 20456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20456-1-AP (EI24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, HT-1080 cells, HeLa cells, L02 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20456-1-AP is EI24 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EI24 fusion protein Ag14169 相同性解析による交差性が予測される生物種
Full Name etoposide induced 2.4 mRNA
Calculated molecular weight 340 aa, 39 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC002390
Gene symbol EI24
Gene ID (NCBI) 9538
RRIDAB_10858326
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ei24 is an ER-localized protein originally identified as a p53-induced proapoptotic gene in etoposide-treated NIH3T3 cells. It has been shown to be able to bind to the antiapoptotic protein Bcl-2 (PMID: 15781622), play a role in autophagy (PMID: 23074225), and induce proliferate arrest/apoptosis (PMID: 10594026). Ei24 can bind specifically to IMPβ1 and IMPα2 to impede their normal role in nuclear import (PMID: 24821838).

Protocols

Product Specific Protocols
WB protocol for EI24 antibody 20456-1-APDownload protocol
IHC protocol for EI24 antibody 20456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Int J Biochem Cell Biol

Etoposide-induced protein 2.4 ameliorates high glucose-induced epithelial-mesenchymal transition by activating adenosine monophosphate-activated protein kinase pathway in renal tubular cells

Authors - Fan Li
  • KD Validated
View Article
humanWB

BMC Cancer

Circular RNA hsa_circ_0043278 inhibits breast cancer progression via the miR-455-3p/EI24 signalling pathway

Authors - Yue Shi
  • KD Validated
View Article
humanWB,IHC

Expert Rev Mol Diagn

Prognostic role and immune infiltration characteristics of EI24 in multiple cancer types

Authors - Wanli Yang
View Article