Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-1080 cells, U-937 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
11734-1-AP targets EID1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EID1 fusion protein Ag2336 相同性解析による交差性が予測される生物種 |
| Full Name | EP300 interacting inhibitor of differentiation 1 |
| Calculated molecular weight | 187 aa, 21 kDa |
| Observed molecular weight | 20-30 kDa |
| GenBank accession number | BC024151 |
| Gene Symbol | EID1 |
| Gene ID (NCBI) | 23741 |
| RRID | AB_10640804 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6B2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EID1(EP300 interacting inhibitor of differentiation 1) is a member of the EID protein family, which regulates differentiation, transcription and acetyltransferase activity. EID1 function was originally identified as a transcriptional repressor which regulates cell cycle and differentiation, and leads to genome inactivation and instability by interacting with E3 ubiquitin ligase MDM2. EID1 is mainly located in the cytoplasm. It was translocated into the nuclei under stressed or pathological conditions such as AD patients and APP Tg mice brains.(PMID: 22186421, PMID: 31381951,PMID: 30926163).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EID1 antibody 11734-1-AP | Download protocol |
| IHC protocol for EID1 antibody 11734-1-AP | Download protocol |
| IF protocol for EID1 antibody 11734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A A comprehensive method for detecting ubiquitinated substrates using TR-TUBE. | ||
Mol Cell Proteomics Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924. | ||
Folia Histochem Cytobiol EID1 plays a protective role in early-onset pre-eclampsia via promoting proliferation and invasion in trophoblast cells. | ||