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EID1 Polyclonal antibody

EID1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11734-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

21 kDa pRb-associated protein, C15orf3, CRI 1, CRI1, E1A-like inhibitor of differentiation 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHT-1080 cells, U-937 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11734-1-AP targets EID1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EID1 fusion protein Ag2336 相同性解析による交差性が予測される生物種
Full Name EP300 interacting inhibitor of differentiation 1
Calculated molecular weight 187 aa, 21 kDa
Observed molecular weight 20-30 kDa
GenBank accession numberBC024151
Gene Symbol EID1
Gene ID (NCBI) 23741
RRIDAB_10640804
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6B2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EID1(EP300 interacting inhibitor of differentiation 1) is a member of the EID protein family, which regulates differentiation, transcription and acetyltransferase activity. EID1 function was originally identified as a transcriptional repressor which regulates cell cycle and differentiation, and leads to genome inactivation and instability by interacting with E3 ubiquitin ligase MDM2. EID1 is mainly located in the cytoplasm. It was translocated into the nuclei under stressed or pathological conditions such as AD patients and APP Tg mice brains.(PMID: 22186421, PMID: 31381951,PMID: 30926163).

Protocols

Product Specific Protocols
WB protocol for EID1 antibody 11734-1-APDownload protocol
IHC protocol for EID1 antibody 11734-1-APDownload protocol
IF protocol for EID1 antibody 11734-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

A comprehensive method for detecting ubiquitinated substrates using TR-TUBE.

Authors - Yukiko Yoshida
humanWB

Mol Cell Proteomics

Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924.

Authors - Hua Liao
humanWB,IHC

Folia Histochem Cytobiol

EID1 plays a protective role in early-onset pre-eclampsia via promoting proliferation and invasion in trophoblast cells.

Authors - Ying Li
WB

Mol Cell

Sugar-mediated non-canonical ubiquitination impairs Nrf1/NFE2L1 activation

Authors - Yukiko Yoshida
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