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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF1AX Polyclonal antibody (14654-1-AP)

    WB analysis using 14654-1-AP (same clone as 14654-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using EIF1AX Polyclonal antibody (14654-1-AP)

    WB analysis of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using EIF1AX Polyclonal antibody (14654-1-AP)

    WB analysis of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using EIF1AX Polyclonal antibody (14654-1-AP)

    WB analysis of A375 using 14654-1-AP (same clone as 14654-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF1AX Polyclonal antibody (14654-1-AP)

    IF Staining of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF1AX antibody (14654-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

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EIF1AX Polyclonal antibody, PBS Only

EIF1AX Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 14654-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14654-1-PBS targets EIF1AX in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF1AX fusion protein Ag6253 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 1A, X-linked
Calculated molecular weight 16 kDa
Observed molecular weight 16-23 kDa
GenBank accession numberBC067851
Gene Symbol EIF1AX
Gene ID (NCBI) 1964
RRIDAB_2878072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47813
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.