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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF1, EIF1B Polyclonal antibody

EIF1, EIF1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15887-1-AP

Synonyms

EIF1B, GC20

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using EIF1, EIF1B Polyclonal antibody (15887-1-AP)

    WB analysis of L02 using 15887-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15887-1-AP (EIF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EIF1, EIF1B Polyclonal antibody (15887-1-AP)

    WB analysis of HepG2 using 15887-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15887-1-AP (EIF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EIF1, EIF1B Polyclonal antibody (15887-1-AP)

    WB analysis of HeLa using 15887-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15887-1-AP (EIF1B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF1, EIF1B Polyclonal antibody (15887-1-AP)

    IHC staining of human kidney using 15887-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15887-1-AP (EIF1B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF1, EIF1B Polyclonal antibody (15887-1-AP)

    IHC staining of human kidney using 15887-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15887-1-AP (EIF1B Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inL02 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15887-1-AP targets EIF1, EIF1B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF1, EIF1B fusion protein Ag8673 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 1B
Calculated molecular weight 113 aa, 13 kDa
Observed molecular weight14 kDa
GenBank accession numberBC006996
Gene symbol EIF1B
Gene ID (NCBI) 10289
RRIDAB_2878196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF1, EIF1B antibody 15887-1-APDownload protocol
IHC protocol for EIF1, EIF1B antibody 15887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols