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KD/KO Validated

EIF2AK1 Polyclonal antibody

Name: EIF2AK1 Polyclonal antibody
Brand: Proteintech
SKU: 20499-1-AP

EIF2AK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20499-1-AP

Synonyms

Heme-controlled repressor, Heme regulated inhibitor, Heme controlled repressor, HCR, Eukaryotic translation initiation factor 2-alpha kinase 1

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 20499-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20499-1-AP
WB result of EIF2AK1 antibody (20499-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells.
WB analysis of HepG2 using 20499-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 20499-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 20499-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 20499-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2AK1 antibody (20499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 20499-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, MCF-7 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 17 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

20499-1-AP targets EIF2AK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EIF2AK1 fusion protein Ag14236 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 2-alpha kinase 1
Calculated molecular weight	630 aa, 71 kDa
Observed molecular weight	71 kDa
GenBank accession number	BC006524
Gene symbol	EIF2AK1
Gene ID (NCBI)	27102
RRID	AB_10697665
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

Protocols

Product Specific Protocols
WB protocol for EIF2AK1 antibody 20499-1-AP	Download protocol
IHC protocol for EIF2AK1 antibody 20499-1-AP	Download protocol
IF protocol for EIF2AK1 antibody 20499-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Nature Stress response silencing by an E3 ligase mutated in neurodegeneration Authors - Diane L Haakonsen View Article
human	WB,CoIP	Nature A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol. Authors - Evelyn Fessler View Article
human	WB	Science Human NLRP1 is a sensor for double-stranded RNA. Authors - Stefan Bauernfried View Article
human	WB	Mol Cell Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis Authors - Yi Fu KD Validated View Article
human	WB	Mol Cell The HRI branch of the integrated stress response selectively triggers mitophagy Authors - Yogaditya Chakrabarty KD Validated View Article
human	WB	Mol Cell Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication. Authors - Jack Chun-Chieh Hsu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF2AK1 Polyclonal antibody

EIF2AK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 20499-1-AP

WB analysis of HEK-293 using 20499-1-AP

WB analysis of HepG2 using 20499-1-AP

IHC staining of human breast cancer using 20499-1-AP

IHC staining of human breast cancer using 20499-1-AP

IF Staining of HepG2 using 20499-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Stress response silencing by an E3 ligase mutated in neurodegeneration

A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Human NLRP1 is a sensor for double-stranded RNA.

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

The HRI branch of the integrated stress response selectively triggers mitophagy

Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication.