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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF2AK1 Monoclonal antibody (67674-1-Ig)

    WB analysis using 67674-1-Ig (same clone as 67674-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EIF2AK1 Monoclonal antibody (67674-1-Ig)

    WB analysis using 67674-1-Ig (same clone as 67674-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF2AK1 Monoclonal antibody (67674-1-Ig)

    IHC staining of human breast cancer using 67674-1-Ig (same clone as 67674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF2AK1 Monoclonal antibody (67674-1-Ig)

    IHC staining of human breast cancer using 67674-1-Ig (same clone as 67674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF2AK1 Monoclonal antibody (67674-1-Ig)

    IF Staining of HeLa using 67674-1-Ig (same clone as 67674-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2AK1 antibody (67674-1-Ig, Clone: 2H1F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

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EIF2AK1 Monoclonal antibody, PBS Only

EIF2AK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67674-1-PBS
Clone No.2H1F3

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67674-1-PBS targets EIF2AK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF2AK1 fusion protein Ag16970 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 1
Calculated molecular weight 630 aa, 71 kDa
Observed molecular weight71 kDa
GenBank accession numberBC006524
Gene Symbol EIF2AK1
Gene ID (NCBI) 27102
RRIDAB_2882869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BQI3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.