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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF2AK2, PKR Monoclonal antibody (66646-1-Ig)

    WB analysis using 66646-1-Ig (same clone as 66646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using EIF2AK2, PKR Monoclonal antibody (66646-1-Ig)

    IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using EIF2AK2, PKR Monoclonal antibody (66646-1-Ig)

    IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF2AK2, PKR Monoclonal antibody (66646-1-Ig)

    IHC staining of human kidney using 66646-1-Ig (same clone as 66646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF2AK2, PKR Monoclonal antibody (66646-1-Ig)

    IHC staining of human kidney using 66646-1-Ig (same clone as 66646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

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EIF2AK2, PKR Monoclonal antibody, PBS Only

EIF2AK2, PKR Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66646-1-PBS
Clone No.2A8A4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

EIF2AK2, PKR, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, EC:2.7.11.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66646-1-PBS targets EIF2AK2, PKR in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF2AK2, PKR fusion protein Ag13010 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 2
Calculated molecular weight 551 aa, 62 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC093676
Gene Symbol EIF2AK2
Gene ID (NCBI) 5610
RRIDAB_2882004
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19525
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.