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EIF2B1 Polyclonal antibody

Name: EIF2B1 Polyclonal antibody
Brand: Proteintech
SKU: 18010-1-AP

EIF2B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18010-1-AP

Synonyms

Translation initiation factor eIF2B subunit alpha, EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of MCF-7 using 18010-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 18010-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18010-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 18010-1-AP
IP result of anti-EIF2B1 (IP:18010-1-AP, 4ug; Detection:18010-1-AP 1:700) with K-562 cells lysate 3200ug.
IHC staining of mouse brain using 18010-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 18010-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 18010-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18010-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18010-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18010-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 18010-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2B1 antibody (18010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 18010-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF7 cells, HeLa cells, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse brain tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

18010-1-AP targets EIF2B1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EIF2B1 fusion protein Ag12509 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
Calculated molecular weight	305 aa, 34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC103763
Gene symbol	EIF2B1
Gene ID (NCBI)	1967
RRID	AB_2261996
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF2B1 antibody 18010-1-AP	Download protocol
IHC protocol for EIF2B1 antibody 18010-1-AP	Download protocol
IF protocol for EIF2B1 antibody 18010-1-AP	Download protocol
IP protocol for EIF2B1 antibody 18010-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Nat Microbiol Inhibition of the integrated stress response by viral proteins that block p-eIF2-eIF2B association. Authors - Huib H Rabouw View Article
human	WB	Nat Commun Sugar phosphate activation of the stress sensor eIF2B. Authors - Qi Hao View Article
human	WB	Elife Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response. Authors - Carmela Sidrauski View Article
	WB	Elife The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes. Authors - Yao Liang Wong View Article
human	WB	Elife eIF2B conformation and assembly state regulate the integrated stress response. Authors - Michael Schoof View Article
human, mouse	WB	FASEB J Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation. Authors - Noel C Wortham View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF2B1 Polyclonal antibody

EIF2B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of MCF-7 using 18010-1-AP

WB analysis of K-562 using 18010-1-AP

WB analysis of HeLa using 18010-1-AP

IP experiment of K-562 using 18010-1-AP

IHC staining of mouse brain using 18010-1-AP

IHC staining of human breast cancer using 18010-1-AP

IHC staining of human breast cancer using 18010-1-AP

IHC staining of mouse brain using 18010-1-AP

IHC staining of mouse brain using 18010-1-AP

IHC staining of mouse brain using 18010-1-AP

IF Staining of HeLa using 18010-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Inhibition of the integrated stress response by viral proteins that block p-eIF2-eIF2B association.

Sugar phosphate activation of the stress sensor eIF2B.

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.

eIF2B conformation and assembly state regulate the integrated stress response.

Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.