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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF2B3 Polyclonal antibody, PBS Only

EIF2B3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11296-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF2B3 Polyclonal antibody (11296-2-AP)

    WB analysis using 11296-2-AP (same clone as 11296-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11296-2-AP (EIF2B3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11296-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EIF2B3 Polyclonal antibody (11296-2-AP)

    IHC staining of mouse colon using 11296-2-AP (same clone as 11296-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11296-2-AP (EIF2B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11296-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EIF2B3 Polyclonal antibody (11296-2-AP)

    IHC staining of mouse colon using 11296-2-AP (same clone as 11296-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11296-2-AP (EIF2B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11296-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF2B3 Polyclonal antibody (11296-2-AP)

    IF Staining of HeLa using 11296-2-AP (same clone as 11296-2-PBS)

    Immunofluorescent analysis of HeLa cells using 11296-2-AP (EIF2B3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11296-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11296-2-PBS targets EIF2B3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2B3 fusion protein Ag1809 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa
Calculated molecular weight 44 kDa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC018728
Gene symbol EIF2B3
Gene ID (NCBI) 8891
RRIDAB_2096142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.