Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, K-562 cells, HeLa cells, HL-60 cells, mouse skeletal muscle tissue |
| Positive IHC detected in | human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
Product Information
11332-1-AP targets EIF2B4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EIF2B4 fusion protein Ag1875 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa |
| Calculated molecular weight | 523 aa, 58 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | BC001870 |
| Gene Symbol | EIF2B4 |
| Gene ID (NCBI) | 8890 |
| RRID | AB_2277640 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI10 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| IHC protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| IF protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell The HRI branch of the integrated stress response selectively triggers mitophagy
| ||
Elife Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response. | ||
Elife The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes. | ||
ACS Cent Sci Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b. | ||