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  • KD/KO Validated

EIF2B4 Polyclonal antibody

EIF2B4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11332-1-AP

Synonyms

Translation initiation factor eIF2B subunit delta, EIF2Bdelta, EIF2BD, eIF2B GDP-GTP exchange factor subunit delta, EIF2B


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Tested Applications

Positive WB detected inMCF-7 cells, K-562 cells, HeLa cells, HL-60 cells, mouse skeletal muscle tissue
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11332-1-AP targets EIF2B4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2B4 fusion protein Ag1875 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
Calculated molecular weight 523 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC001870
Gene symbol EIF2B4
Gene ID (NCBI) 8890
RRIDAB_2277640
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF2B4 antibody 11332-1-APDownload protocol
IHC protocol for EIF2B4 antibody 11332-1-APDownload protocol
IF protocol for EIF2B4 antibody 11332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

The HRI branch of the integrated stress response selectively triggers mitophagy

Authors - Yogaditya Chakrabarty
  • KD Validated
humanWB

Nat Commun

Sugar phosphate activation of the stress sensor eIF2B.

Authors - Qi Hao
humanWB

Elife

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

Authors - Carmela Sidrauski
WB

Elife

The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.

Authors - Yao Liang Wong
humanWB

ACS Cent Sci

Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b.

Authors - Xiaoyu Zhang
humanWB

Elife

eIF2B conformation and assembly state regulate the integrated stress response.

Authors - Michael Schoof