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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF2C1-Specific Polyclonal antibody

EIF2C1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19690-1-AP

Synonyms

AGO1, Argonaute1, eIF 2C 1, EIF2C, eIF2C 1, EIF2C1, EIF2C1-Specific, GERP95, hAgo1, Protein argonaute 1, Q99

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    WB analysis of HeLa using 19690-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    WB analysis of HeLa using 19690-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    WB analysis of HeLa using 19690-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    WB analysis of NIH/3T3 using 19690-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    WB analysis of Jurkat using 19690-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    WB analysis of HEK-293 using 19690-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    WB analysis of K-562 using 19690-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    IHC staining of human cervical cancer using 19690-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    IHC staining of human cervical cancer using 19690-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    IHC staining of human ovary tumor using 19690-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    IHC staining of human ovary tumor using 19690-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    IF Staining of HepG2 using 19690-1-AP

    Immunofluorescent analysis of HepG2 cells using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF2C1-Specific Polyclonal antibody (19690-1-AP)

    IF Staining of HepG2 using 19690-1-AP

    Immunofluorescent analysis of HepG2 cells using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman cervical cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19690-1-AP targets EIF2C1-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2C, 1
Calculated molecular weight 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberNM_012199
Gene symbol EIF2C1
Gene ID (NCBI) 26523
RRIDAB_10863657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF2C1, also named as AGO1, belongs to the argonaute family and Ago subfamily. EIF2C1 is required for RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. EIF2C1 may also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). The antibody is specific to EIF2C1.

Protocols

Product Specific Protocols
WB protocol for EIF2C1-Specific antibody 19690-1-APDownload protocol
IHC protocol for EIF2C1-Specific antibody 19690-1-APDownload protocol
IF protocol for EIF2C1-Specific antibody 19690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Mol Neurosci

miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif.

Authors - Belinda J Goldie
View Article
humanWB

Mol Ther Oncolytics

Hsa_circ_0001666 promotes non-small cell lung cancer migration and invasion through miR-1184/miR-548I/AGO1 axis.

Authors - Xueting Wang
View Article
mouseIF

bioRxiv

MicroRNAs are necessary for the emergence of Purkinje cell identity

Authors - Norjin Zolboot
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Roy (Verified Customer) (06-15-2024)

Unfortunately it did detect both Ago1 and Ago2 (validation from siRNA experiments)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa