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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF2C3-Specific Polyclonal antibody

EIF2C3-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19692-1-AP

Synonyms

AGO3, Argonaute 3, Argonaute3, eIF 2C 3, eIF2C 3, EIF2C3, EIF2C3-Specific, hAgo3, Protein argonaute 3

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using EIF2C3-Specific Polyclonal antibody (19692-1-AP)

    WB analysis of SKOV-3 using 19692-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EIF2C3-Specific Polyclonal antibody (19692-1-AP)

    WB analysis of HepG2 using 19692-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EIF2C3-Specific Polyclonal antibody (19692-1-AP)

    WB analysis of HepG2 using 19692-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EIF2C3-Specific Polyclonal antibody (19692-1-AP)

    IHC staining of human stomach cancer using 19692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19692-1-AP (EIF2C3-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EIF2C3-Specific Polyclonal antibody (19692-1-AP)

    IHC staining of human stomach cancer using 19692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19692-1-AP (EIF2C3-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSKOV-3 cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

19692-1-AP targets EIF2C3-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2C, 3
Calculated molecular weight 97 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberNM_024852
Gene symbol EIF2C3
Gene ID (NCBI) 192669
RRIDAB_2878597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF2C3, also named as AGO3, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi). EIF2C3 binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. EIF2C3 lacks endonuclease activity and does not appear to cleave target mRNAs. This antibody is specific to EIF2C3.

Protocols

Product Specific Protocols
WB protocol for EIF2C3-Specific antibody 19692-1-APDownload protocol
IHC protocol for EIF2C3-Specific antibody 19692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

A novel class of microRNA-recognition elements that function only within open reading frames.

Authors - Kai Zhang
View Article
humanWB

Front Cell Infect Microbiol

Argonaute-2 autoantibodies: a promising biomarker for predicting mortality in HBV-related acute-on-chronic liver failure patients with cirrhosis

Authors - Yixuan Wang
View Article