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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF2S1 Recombinant antibody

EIF2S1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230245A12

Cat no : 82936-1-RR

Synonyms

EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A, eIF 2 alpha

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EIF2S1 Recombinant antibody (82936-1-RR)

    WB analysis using 82936-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF2S1 Recombinant antibody (82936-1-RR)

    WB analysis using 82936-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF2S1 Recombinant antibody (82936-1-RR)

    WB analysis using 82936-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF2S1 Recombinant antibody (82936-1-RR)

    WB analysis using 82936-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using EIF2S1 Recombinant antibody (82936-1-RR)

    IP experiment of HepG2 using 82936-1-RR

    IP result of anti-EIF2S1 (IP:82936-1-RR, 4ug; Detection:82936-1-RR 1:3000) with HepG2 cells lysate 1470 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EIF2S1 Recombinant antibody (82936-1-RR)

    IHC staining of mouse brain using 82936-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82936-1-RR (EIF2S1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EIF2S1 Recombinant antibody (82936-1-RR)

    IHC staining of mouse brain using 82936-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82936-1-RR (EIF2S1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF2S1 Recombinant antibody (82936-1-RR)

    IF Staining of HepG2 using 82936-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2S1 antibody (82936-1-RR, Clone: 230245A12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of MCF-7 cells using EIF2S1 Recombinant antibody (82936-1-RR)

    FC experiment of MCF-7 using 82936-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using EIF2S1 Recombinant antibody (82936-1-RR)

    FC experiment of HeLa using 82936-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82936-1-RR

    Affinity and Kinetic Characterization of 82936-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82936-1-RR against Human EIF2S1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, A549 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82936-1-RR targets EIF2S1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EIF2S1 fusion protein Ag1645 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002513
Gene symbol EIF2S1
Gene ID (NCBI) 1965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.

Protocols

Product Specific Protocols
WB protocol for EIF2S1 antibody 82936-1-RRDownload protocol
IHC protocol for EIF2S1 antibody 82936-1-RRDownload protocol
IF protocol for EIF2S1 antibody 82936-1-RRDownload protocol
IP protocol for EIF2S1 antibody 82936-1-RRDownload protocol
FC protocol for EIF2S1 antibody 82936-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols