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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF2S2 Monoclonal antibody (68463-1-Ig)

    WB analysis using 68463-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68463-1-Ig (EIF2S2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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EIF2S2 Monoclonal antibody

EIF2S2 Monoclonal Antibody for WB, ELISA
Cat No. 68463-1-Ig
Clone No.2H8E1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

DKFZp686L18198, eIF 2 beta, EIF2, EIF2B, EIF2beta, EIF2S2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68463-1-Ig targets EIF2S2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF2S2 fusion protein Ag18349 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa
Calculated molecular weight 38 kDa
Observed molecular weight50 kDa
GenBank accession numberBC000461
Gene Symbol EIF2S2
Gene ID (NCBI) 8894
RRIDAB_3085174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G Magarose purification
UNIPROT IDP20042
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation.

Protocols

Product Specific Protocols
WB protocol for EIF2S2 antibody 68463-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols