EIF2S3 Polyclonal antibody

Name: EIF2S3 Polyclonal antibody
Brand: Proteintech
SKU: 11162-1-AP

EIF2S3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11162-1-AP

Synonyms

EIF2G, EIF2, eIF 2gX, eIF 2gA, eIF 2 gamma X

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 11162-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 11162-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 11162-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11162-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 11162-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11162-1-AP
IP result of anti-EIF2S3 (IP:11162-1-AP, 4ug; Detection:11162-1-AP 1:500) with HeLa cells lysate 2800ug.
IHC staining of human lung cancer using 11162-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11162-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11162-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 11162-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human tonsillitis using 11162-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human tonsillitis using 11162-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 11162-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 11162-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 11162-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 11162-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse thymus tissue, COLO 320 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below

Product Information

11162-1-AP targets EIF2S3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EIF2S3 fusion protein Ag1650 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
Calculated molecular weight	52 kDa
Observed molecular weight	52 kDa
GenBank accession number	BC019906
Gene symbol	EIF2S3
Gene ID (NCBI)	1968
RRID	AB_2246331
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Interacts with GTP and initiator methionyl-tRNA, translation initiation factor eIF2 forms a ternary complex that binds the 40S ribosome and then scans an mRNA to select the AUG start codon for protein synthesis[PMID: 9736774]. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S3 is the core subunit of the heterotrimeric eIF2 complex.[PMID:23063529]

Protocols

Product Specific Protocols
WB protocol for EIF2S3 antibody 11162-1-AP	Download protocol
IHC protocol for EIF2S3 antibody 11162-1-AP	Download protocol
IF protocol for EIF2S3 antibody 11162-1-AP	Download protocol
IP protocol for EIF2S3 antibody 11162-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins Authors - Yulin Chen View Article
human	WB	Nat Neurosci Deletion of TOP3β, a component of FMRP-containing mRNPs, contributes to neurodevelopmental disorders. Authors - Stoll Georg G View Article
human	WB	Cell Rep RNA helicase IGHMBP2 regulates THO complex to ensure cellular mRNA homeostasis Authors - Archana Bairavasundaram Prusty View Article
human	WB	Front Biosci (Landmark Ed) EIF2Ss, a Novel c-Myc-Correlated Gene Family, is Associated with Poor Prognosis and Immune Infiltration in Pancreatic Adenocarcinoma Authors - Zhangqi Cao View Article
human	WB	Mol Biol Cell PRMT7 Methylates Eukaryotic Translation Initiation Factor 2α and Regulates its Role in Stress Granule Formation. Authors - Nasim Haghandish View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF2S3 Polyclonal antibody

EIF2S3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 11162-1-AP

WB analysis of mouse thymus using 11162-1-AP

WB analysis of mouse thymus using 11162-1-AP

WB analysis of HeLa using 11162-1-AP

WB analysis of COLO 320 using 11162-1-AP

IP experiment of HeLa using 11162-1-AP

IHC staining of human lung cancer using 11162-1-AP

IHC staining of human lung cancer using 11162-1-AP

IHC staining of human lung cancer using 11162-1-AP

IHC staining of human lung cancer using 11162-1-AP

IHC staining of human tonsillitis using 11162-1-AP

IHC staining of human tonsillitis using 11162-1-AP

IHC staining of human lung cancer using 11162-1-AP

IHC staining of human lung cancer using 11162-1-AP

IF Staining of HeLa using 11162-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Deletion of TOP3β, a component of FMRP-containing mRNPs, contributes to neurodevelopmental disorders.

RNA helicase IGHMBP2 regulates THO complex to ensure cellular mRNA homeostasis

EIF2Ss, a Novel c-Myc-Correlated Gene Family, is Associated with Poor Prognosis and Immune Infiltration in Pancreatic Adenocarcinoma

PRMT7 Methylates Eukaryotic Translation Initiation Factor 2α and Regulates its Role in Stress Granule Formation.