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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF3A Polyclonal antibody

EIF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27665-1-AP

Synonyms

eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180, eIF3 p185, eIF3 theta, EIF3A, EIF3S10, KIAA0139, P167, p180, p185, TIF32

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using EIF3A Polyclonal antibody (27665-1-AP)

    WB analysis of BxPC-3 using 27665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27665-1-AP (EIF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF3A Polyclonal antibody (27665-1-AP)

    WB analysis using 27665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27665-1-AP (EIF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EIF3A Polyclonal antibody (27665-1-AP)

    IHC staining of human lung cancer using 27665-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27665-1-AP (EIF3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EIF3A Polyclonal antibody (27665-1-AP)

    IHC staining of human lung cancer using 27665-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27665-1-AP (EIF3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF3A Polyclonal antibody (27665-1-AP)

    IF Staining of HepG2 using 27665-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 27665-1-AP (EIF3A antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inBxPC-3 cells, SKOV-3 cells, mouse brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27665-1-AP targets EIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3A fusion protein Ag24752 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit A
Calculated molecular weight 1382 aa, 166 kDa
Observed molecular weight 166 kDa
GenBank accession numberBC114429
Gene symbol EIF3A
Gene ID (NCBI) 8661
RRIDAB_2880942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF3A antibody 27665-1-APDownload protocol
IHC protocol for EIF3A antibody 27665-1-APDownload protocol
IF protocol for EIF3A antibody 27665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols