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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF3C Polyclonal antibody

EIF3C Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12733-1-AP

Synonyms

EIF3S8, EIF3CL, eIF3 p110

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using EIF3C Polyclonal antibody (12733-1-AP)

    WB analysis of K-562 using 12733-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12733-1-AP (EIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using EIF3C Polyclonal antibody (12733-1-AP)

    WB analysis of MCF-7 using 12733-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12733-1-AP (EIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using EIF3C Polyclonal antibody (12733-1-AP)

    IP experiment of K-562 using 12733-1-AP

    IP result of anti-EIF3C (IP:12733-1-AP, 4ug; Detection:12733-1-AP 1:500) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EIF3C Polyclonal antibody (12733-1-AP)

    IHC staining of human stomach cancer using 12733-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 12733-1-AP (EIF3C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EIF3C Polyclonal antibody (12733-1-AP)

    IHC staining of human colon cancer using 12733-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12733-1-AP (EIF3C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EIF3C Polyclonal antibody (12733-1-AP)

    IHC staining of human colon cancer using 12733-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12733-1-AP (EIF3C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12733-1-AP targets EIF3C in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3C fusion protein Ag3438 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit C
Calculated molecular weight 913 aa, 110 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC001571
Gene symbol EIF3C
Gene ID (NCBI) 8663
RRIDAB_2096747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF3C antibody 12733-1-APDownload protocol
IHC protocol for EIF3C antibody 12733-1-APDownload protocol
IP protocol for EIF3C antibody 12733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression

Authors - Yanan Pi
View Article