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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF3D Monoclonal antibody, PBS Only

EIF3D Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C5A3

Cat no : 66024-1-PBS

Synonyms

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using EIF3D Monoclonal antibody (66024-1-Ig)

    WB analysis of HepG2 using 66024-1-Ig (same clone as 66024-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using EIF3D Monoclonal antibody (66024-1-Ig)

    WB analysis of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF3D Monoclonal antibody (66024-1-Ig)

    WB analysis of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using EIF3D Monoclonal antibody (66024-1-Ig)

    WB analysis of A431 using 66024-1-Ig (same clone as 66024-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF3D Monoclonal antibody (66024-1-Ig)

    WB analysis of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using EIF3D Monoclonal antibody (66024-1-Ig)

    WB analysis of mouse liver using 66024-1-Ig (same clone as 66024-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using EIF3D Monoclonal antibody (66024-1-Ig)

    WB analysis of rat liver using 66024-1-Ig (same clone as 66024-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using EIF3D Monoclonal antibody (66024-1-Ig)

    IHC staining of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using EIF3D Monoclonal antibody (66024-1-Ig)

    IHC staining of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF3D Monoclonal antibody (66024-1-Ig)

    IF Staining of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66024-1-PBS targets EIF3D in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EIF3D fusion protein Ag18092 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit D
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC000328
Gene symbol EIF3D
Gene ID (NCBI) 8664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.