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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF3D Recombinant antibody, PBS Only

EIF3D Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3O8

Cat no : 82512-1-PBS

Synonyms

eIF-3-zeta, EIF3S7, eIF3 zeta, eIF3 p66, eIF 3 zeta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF3D Recombinant antibody (82512-1-RR)

    WB analysis using 82512-1-RR (same clone as 82512-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82512-1-RR (EIF3D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using EIF3D Recombinant antibody (82512-1-RR)

    WB analysis of rat liver using 82512-1-RR (same clone as 82512-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 82512-1-RR (EIF3D antibody) at dilution of 1:30300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using EIF3D Recombinant antibody (82512-1-RR)

    IP experiment of A549 using 82512-1-RR (same clone as 82512-1-PBS)

    IP result of anti-EIF3D (IP:82512-1-RR, 4ug; Detection:82512-1-RR 1:5000) with A549 cells lysate 920 ug. This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF3D Recombinant antibody (82512-1-RR)

    IHC staining of human breast cancer using 82512-1-RR (same clone as 82512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82512-1-RR (EIF3D antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF3D Recombinant antibody (82512-1-RR)

    IHC staining of human breast cancer using 82512-1-RR (same clone as 82512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82512-1-RR (EIF3D antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EIF3D Recombinant antibody (82512-1-RR)

    IF Staining of U2OS using 82512-1-RR (same clone as 82512-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using EIF3D antibody (82512-1-RR, Clone: 3O8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EIF3D Recombinant antibody (82512-1-RR)

    IF Staining of U2OS using 82512-1-RR (same clone as 82512-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using EIF3D antibody (82512-1-RR, Clone: 3O8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82512-1-RR

    Affinity and Kinetic Characterization of 82512-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82512-1-RR against Human EIF3D were performed. The affinity constant is 5.83 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82512-1-PBS targets EIF3D in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EIF3D fusion protein Ag0268 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit D
Calculated molecular weight 66 kDa
Observed molecular weight65 kDa
GenBank accession numberBC000328
Gene symbol EIF3D
Gene ID (NCBI) 8664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.